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argument in how to get motivated to write, writing 2017 Steve Campsall. write a more effective argument. Download free revision guides. The word argument brings to mind an animated disagreement between two people, but for your English homework or exams, a written argument is very different. Writing to argue requires that you. put forward a well-considered point of view, stated briefly; provide support for this view; create a sense of balance by beauty vs european beauty referring to one or more other viewpoints; tactfully counter these. Your aim in this kind of to write a paper, writing is not necessarily to digi yellow man 'win' the argument; instead, it is to put forward evidence that is logical and how to get motivated to write well - considered and which acts to support your point of view and to Malcom X's The Ballot of the Bullet examples counter the main alternative views. How To To Write A Paper. The evidence you provide must be both convincing and blood presented convincingly. The evidence needs to be convincing but, in how to, an exam situation at least, it does not have to be factual , i.e. you can 'make it up'; you are allowed to make up such things as expert opinions and when did the uss maine statistical evidence to support your argument. Importantly, whatever evidence you do use, it must be well considered and reasonable . How To To Write A Paper. Remember - whilst you might not agree with an opposing view, that doesn't make it in any way foolish to hold. You will need to take great care indeed to avoid suggesting that those who hold different views are in any way foolish for digi yellow man, doing so. This is such an easy pitfall that catches out very many students.

In large part, it is the degree of politeness and tact that you display when opposing other viewpoints that will win or lose your argument - and gain you the most marks! ARGUMENT OR PERSUASION - what is the difference? 'Writing to argue' and 'writing to persuade ' both occur on a paper school courses. They are both very similar in as much as they share the same purpose , that of seeking to when uss maine influence . There are differences that will affect the style of your writing if you are to gain the how to highest marks. An argument concerns an issue about which people, quite reasonably , hold different views. This suggests that other views are not necessarily wrong - just different . During the process of presenting your argument, therefore, it is reasonable that you should show that you recognise that opposing views exist , not only to hint at what a fair-minded person you are , but to give you the opportunity to counter these views tactfully in digi yellow man, order to show why you feel that your own view is the more worthy one to hold . Persuasion has a more single-minded goal . To Write A Paper. It is based on a personal conviction that a particular way of thinking is the only sensible way to digi yellow man think. WELL-REASONED ARGUMENT vs . How To. PASSIONATE PERSUASION!

Consider this typical scene in a teenager's life. Essay. The party is on Friday. and, naturally, you really want to go but your parents have other ideas. They're planning a visit to Great Aunt Bertha and know how much she'd love to see how you've grown since your last visit. Persuading your mum to say yes to the party is your determined goal - because Friday is the how to get motivated to write a paper deadline and Ballot of the you need an how to get motivated to write, answer now. How to Malcom X's The Bullet Essay examples go about it? First, a little calm reasoning ('Everyone from school will be there, mum. It's a social occasion and it'll help me make more friends. ' ), next a little reasoned anger ('When you were young I bet you went to parties. ') and to write a paper finally, a passionate plea ('Oh, do please try to see it from my position, mum.

I can't turn up on Monday the only kid in the class who didn't go. '). Now if instead of the above, you had been asked to write an article in the school magazine to present a case for a return of end-of-year school disco. well, you don't need that immediate answer, so a well-reasoned argument composed of a series of well thought out and well-supported points is likely to win the day. The pressure is on in the first case, but not in Drug Prevention Programs Essays, the second. A little history will help. ARGUMENT AND THE ANCIENT ART OF 'RHETORIC' If Aristotle and how to get motivated to write Cicero were writing this web page, they would be telling you that the ideal form of argument was through the use of wedding, one thing and get motivated to write one thing alone. reason (which had the Greek name of logos - hence the modern word logic ); however, the two recognised that 'ideal' things must always remain just that - ideas , and wedding that human weakness would always mean that two further argument techniques would be brought into use, especially where persuasion was needed.

The first of these is an appeal to character (which they called ethos - hence our term ethical ) and the second? An appeal to how to get motivated the emotions (which they called pathos - a word we now use to suggest the power to stir sad emotions). Drug Programs. Most people believe themselves to be reasonable, so appealing to a person's sense of reason is likely to be the most effective means of convincing them to change their way of thinking ('If we don't do this. then. ' ). Writing an effective argument. How To Get Motivated To Write. An argument should set out to Scientific answer the how to get motivated a paper question 'Why?' for your viewpoint as well as show awareness and understanding of your opponent's views . The secrets of success? Show you understand the genre conventions of the form - that is, the format - in when sink, which you are asked to write (e.g. an article, a letter, a speech, etc.). Find common ground - an endpoint upon which all would agree . Show consideration of but counter with politeness and tact your opponent's views. How To To Write A Paper. Use effective argumentative techniques - that is, use rhetorical devices. Did The Uss Maine. Ensure your views unfold logically and how to get motivated to write persuasively - that is, create a logical structure for your argument.

Showing understanding of opposing views. It's crucial to blood wedding find some common ground that exists between you - there will always be something worthwhile on how to get motivated which both you and your opponents agree. Think hard about the views your opponents hold and why they hold such views - and always recognise and show that they see that this is blood wedding sparknotes, a fair view to get motivated a paper hold (but not the best view. When. which is your own). Be tactful - never show disrespect for to write, another's point of view. Digi Yellow Man. Sound sincere - give your writing an authentic feel. Sound interested, knowledgeable, trustworthy and convincing. Logic and reason win arguments - but be passionate about your views Avoid sounding superior, condescending or impolite. As made clear already, any suggestion that other viewpoints are 'silly' or 'foolish' is the equivalent of calling your opponent 'silly' and 'foolish'. The result? Lost argument. Lost marks.

Try switching roles - which points would convince you? Showing understanding of form and conventions. You may be asked to write in the form of an essay, a formal or informal letter, a newspaper or magazine article, a school newsletter, a speech, and so on. How To Get Motivated To Write A Paper. Make sure you know the genre conventions that apply to each of these. Using effective argumentative techniques.

Remember that because the X's The Ballot of the other view is reasonably held, you will only win through sensible reasoning, logic and evidence. The key to a successful argument is to plan well, making sure you release your various points in a progressively convincing order. Try to how to get motivated to write a paper show that you have planned or, as the examiner's put it: ' consciously shaped your response '. This gains many extra marks. You need to show that your reader can trust you - so make up a solid and believable reason why you are in a good position to argue over this issue . An effective way of convincing someone that you are reasonable is to find the common ground that exists between you. This is an outcome that is common to Method you both - an how to a paper, end-point you both desire. Ballot Essay. Having acknowledged this, you then proceed by showing how your way to this goal is the best way.

A solid way to seem reasonable is to acknowledge the main opposing view(s) - but to show subtly how your view is more reasonable. Using an interesting - but entirely reasonable and realistic - anecdote to illustrate your main point can be very compelling and powerful, e.g. To Write A Paper. 'Let me tell you about a friend of mine. S/he. '. Use rhetorical devices such as rhetorical questions , 'list of three', repetition, etc. to increase the persuasive power of your argument. Successful arguments are. INTERESTING, ORIGINAL AND APPEALING ORGANISED TO BE PROGRESSIVELY CONVINCING REASONABLE EASY TO FOLLOW THOUGHTFUL AND CONSIDERATE ARGUED FROM A PASSIONATE POSITION SUBTLE and did the sink SOPHISTICATED.

Here is a small section of the mark scheme the get motivated to write examiners from a major examining board use when they award a grade A: #8226; shows sustained awareness of the audience. #8226; arguments are convincingly developed and supported by relevant detail. #8226; ideas are selected and prioritised to construct a sophisticated argument. #8226; paragraphs are effectively varied in length and Malcom of the Bullet Essay structure. #8226; a wide range of appropriate, ambitious vocabulary is used. Planning: put simply, you will lose marks if your argument fails to have an get motivated to write a paper, effective structure . So a clear plan is when did the uss maine sink, essential to how to a paper an effective written argument . The examiner's mark schedule states that your argument needs to blood show evidence of being consciously shaped . Decide on the most likely effective style - should you use standard English (probably. ), be very formal (depends. ), be chatty (probably not. ) and so on. Brainstorm to create a list of points in favour of your idea. Choose five of the most convincing . Check that each point is truly separate and how to a paper not a part of a larger, more general point if it is, use the larger point. Digi Yellow Man. Make sure each point would convince you if you were your opposition! Organise your five points into a progressive and persuasive order. Work out one or two of the main opposing points.

Think how you could add authority to how to to write a paper your writing: a piece of research, an expert opinion, a quotation from a respected source. (always sensible and made up for american beauty vs european beauty, the exam!). Would an anecdote be a persuasive device to use? Remember to use reason but state it passionately if appropriate. Most especially remember that if you as much as even suggest that people are silly or foolish to hold an how to get motivated a paper, opposing view you have lost your argument! And worse. Scientific Method Essay. lost marks. Open strongly and in get motivated to write, an original way to capture your reader's attention. Consider using an anecdote early in the argument. State your own point of view but don't be too strident in your tone . Drug Essays. If relevant and useful, give a little history and a paper background to the argument.

Find some common ground between you and your reader to generate trust and goodwill in you as a person and Scientific Method as a thinker. Get Motivated To Write A Paper. 'Selling' yourself will help your reader decide to 'buy' your ideas. CENTRAL or BODY PARAGRAPHS. Open each paragraph with a topic sentence that introduces the points created from digi yellow man your brainstorm and planning. Write about four to five more sentences that do no more than explain the point made in the topic sentence. Aim to get motivated to write link each paragraph to the next by using a subtle 'transition' or 'hook' sentence at the end of the preceding paragraph. Acknowledge the main opposing view but sensitively refute it to show how your view is more reasonable.

Use rhetorical devices . American Beauty. HERE ARE SOME EXAMPLES OF 'RHETORICAL DEVICES' Rhetorical questions, similes, metaphors, emotive language (use with care!), irony (but never sarcasm!, 'lists of three', repetition, hyperbole (exaggeration for effect), humour, anecdotes, and so on. Help your argument flow by to write a paper using 'discourse markers' . These act as 'signposts' to help your ideas flow and aid your readers journey through your essay. American Beauty Beauty. HERE ARE SOME EXAMPLES OF 'DISCOURSE MARKERS' Add authenticity to your argument by creating a sincere tone , and by using an anecdote . Add authority to get motivated a paper your argument by writing confidently and making up sensible but effective support, e.g. 'expert' opinion, statistics, and so on - make this up in the exam, but keep it reasonable. Round up your argument by restating your case succinctly and summarising your main points. End as you began - in a powerful, interesting and memorable way. Each year, literally thousands of did the, students fail to achieve the to write marks they could. Don't be one of them ALWAYS CHECK YOUR WRITING BEFORE HANDING IN!

Read each sentence immediately after you write it. Read each sentence before you proceed to Prevention Programs Essays the next to check it is fluent, accurate and complete. Does it follow on logically from the previous sentence? Check every paragraph. Avoid creating overly short paragraphs as this suggests either a) you do not know what a paragraph is how to get motivated to write a paper, or b) that you have no explained the point of the paragraph in sufficient detail. Try to make sure that each paragraph flows naturally on from its predecessor by using the final sentence of each paragraph to when sink subtly 'hook' into how to get motivated the topic of the next paragraph. To correct a missed paragraph simply put this mark where you want in to be: // then, in your margin write: // = new paragraph. The examiner will not mark you down for this so long as you have not forgotten all of your paragraphs. Examine each comma.

Look at every apostrophe. And when one thing belongs to another thing or person, this is shown by adding apostrophe+s to the owning noun. So the school's entrance shows that this is 'the entrance of the school' and 'Alan's book' shows this is 'the book of Alan'. Similarly, you go 'to the digi yellow man doctor's' and 'the chemist's' as well as to 'Sainsbury's' and to write a paper 'McDonald's' because you mean 'to the doctor's surgery ', 'the chemist's shop ' and so on. Watch out for it's.

With an apostrophe it only ever means it is or it has , as in blood, ' it's cold ' or ' it's got three toes missing '. If you mean belonging to it , as in its fur is shiny and smooth , no apostrophe is how to to write, used.

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Software Engineer Resume: Guide and a Sample [+20 Examples] Welcome to your new office. There's your brand new Herman Miller chair. Your SSD laptop with vertical and horizontal monitors. Your business credit card. Down the hall is a staff kitchen like something from the Zuckerberg mansion. There's free food. Free laundry.

Free massages. Basically, you've nailed a job at how to, a unicorn company. And it all started with a software engineer resume that stood out Ballot Essay examples, like Linus Torvalds. Now. How can you make that dream a reality? It's simple. Just show that great company how much they need you.

This guide will demonstrate: A professional sample software engineer resume. How to pick the best software developer resume format. Usable examples of resume skills, achievements, and accomplishments. How to build your own software engineer resume template to how to get motivated to write, make applying for jobs a lot easier. Here's a sample resume for a software engineer made using our resume builder. Want to save time and have your resume ready in 5 minutes?

Try our resume builder . It’s fast and easy to use. Plus, you'll get tips and right vs. wrong examples while writing your resume. See +20 resume templates and Drug Programs, create your resume here . What's the Best Format for a Software Engineer Resume? The best example of a software engineer resume is to write, one that gets the interview. Yet recruiters, and especially those at american, unicorn companies, spend only how to get motivated to write a paper six seconds looking at each resume. That's why you must use a resume format that grabs on like a LogMeIn connection and doesn't let go.

Use the reverse-chronological layout. It front-loads your strengths, showing all your best moments at Scientific, a glance. To make it really pop, use clear, legible fonts and plenty of white space. Finally, save your software developer resume in PDF format. That'll keep your layout intact as it travels through cyberspace.

Pro Tip: PDFs are the way to go unless they conflict with the company's Applicant Tracking System. How can you know? Check the job listing. How to Write a Resume Summary or Resume Objective. Many career experts say, When writing a software engineer resume, don't use a resume summary or objective statement. Those career experts are wrong. A generic summary or objective will make recruiters yawn. But a good one will grab hiring managers like a sudo command. Use a resume summary if you've got lots of to write, experience.

Use a resume objective if you're a new graduate or you're switching careers. Let's start with the Prevention Programs, resume summary for a software developer. It's a highlights reel of your best shining moments. But there's a right way and a wrong way to get motivated a paper, do it. Two Senior Software Engineer Resume Summary Examples. Look at the two very different software engineer resume examples below:

Software engineer with 7+ years experience, skilled in Ballot of the, C++, ASP.NET, SQL, Java, Python, Javascript, C#, Perl, and PHP, looking for a position as a software developer for how to get motivated to write a paper Oracle. Okay, it sounds impressive. Method! But it's got a fatal error. How To Get Motivated A Paper! It's just like all the others. No wonder career experts say to Scientific Method Essay, avoid it! Now try this short sample, from how to to write a paper a much better software developer resume: Results-Focused Software Engineer. Energetic software engineer with 7+ years experience developing robust code for high-volume businesses. Increased code-efficiency of XYZ Corp customer portal by 55%. Boosted customer retention by 25%. Spearheaded a team that won the 2016 BOSSIE Award for cloud computing.

An enthusiastic team player and deep creative thinker. Wow, right? So do you need to american beauty beauty, win a BOSSIE to get the job? No. Just sift through your past and find those glowing moments that scream, Hire this genius! Start with a deep, brainstormed file of all your selling points. Then, when writing your software engineer resume summary section, use the how to, 3 or 4 that match the job description. How to Write a Resume Objective for an Entry Level Software Engineer. Maybe your work experience is blood wedding, as thin as a healthcare company firewall.

Don't sweat it. Use a resume objective. It's the way to go for fresh graduates or those looking for a new niche. Use these two very different software engineer resumes as a starting point: Two Entry Level Computer Science Resume Examples. Looking for a job as a software engineer because I've got a degree in it, I have studied 23 programming languages like everyone else, and I'm extremely good, trust me. That's not a resume objective statement.

It's a one-way ticket to the recycle bin. Now of course you won't do that, but if you don't get really specific, that's how it'll read. Think measurable details like in the software developer resume objective below: Team player software engineer with a can-do attitude, phenomenal time management skills, and how to get motivated to write, a strong user focus. Prevention Programs Essays! Has developed several web and mobile apps, including a home security system using Raspberry Pi and how to a paper, a webcam. Wow, right? Specific.

But the real key here is whether those strong points match the job description. If not, flat tire. But what if you don't have even that much experience? I'll explain the fix for that in a minute. Pro Tip: Your resume objective or resume summary is the icing on your job search cake. Write it last, and Malcom X's The, include the best bits from the get motivated, rest of your resume.

Still fuzzy on how to blood wedding, write a professional resume summary or objective for a software engineer resume? See the computer science resume template at the top of this article. Or see our guides: How To Write A Resume Summary: 21 Best Examples You Will See AND +20 Resume Objective Examples - Use Them on Your Resume (Tips) How to how to to write a paper, Describe Your Software Engineer Experience. Let's circle back to our unicorn company job. The one with lots of breaks and free Fitbits and a complimentary Kinesis keyboard. How much do you think experience matters to blood sparknotes, that company?

If you said, A lot, gold star. That's why your software engineer resume's experience section needs to how to to write, sing your programming experience from the rooftops. Put your most recent work first. Showcase 4-5 wins and responsibilities that fit the Malcom Ballot, job offer . Glance at the senior software developer resume samples below. They're for a job opening that values leadership, production, file management, and security.

Senior Software Engineer Resume Examples. July 2015 - July 2017. Responsible for spearheading teams that handled the software development life cycle for 10 client projects, end to how to get motivated a paper, end. Built infrastructure to handle millions of client files in proprietary systems. Blood Wedding Sparknotes! Led the drive to implement the Principle of Least Privilege across 100% of software development staff. How To A Paper! Reduced security breaches by 73% for all customers companywide. Built security into when sink new product features to achieve 100% compliance with industry best practices. See that? The candidate used her experience section to prove she fits the job like a glove. She also used great action words to do it. Now look at another senior software engineering resume example.

July 2015 - July 2017. Responsible for leading teams of software developers. Used Java to implement scalable features for client software. Worked independently to how to to write, meet tight deadlines in a dynamic team environment. Used a solid understanding of web protocols to handle a large workload.

Worked on several security projects. Can you see the problem with that software engineer resume sample? It's generic. No achievements. Yet this is the same person and the same job. In the second resume objective example, she just hasn't figured out the hack yet! If you lack experience, don't fret. It's actually pretty easy to get some. You can do free projects for small businesses. You can contribute to open source projects. You can even come up with your own coding activities at home.

Maybe you've designed a home security app for your apartment. To you it was all in fun. But it might make a hiring manager say, This kid has drive. Which of the two junior software engineer resume samples below look more impressive? June 2016 - present. Received my BA in Software Engineering in 2016. Still trying to build experience. I've sent off 15 software engineer resumes so far.

You can't blame the job candidate for not having experience. But she could have the software engineer experience section below. June 2016 - present. Composed an blood wedding app to allow voice control of lights, garage door, and how to a paper, home thermostat, using an sparknotes Amazon Echo and Raspberry Pi. Built a real time stock picking app that compares current price to Uniform Adjusted Financial Reporting Standards data to to write a paper, identify buy-now opportunities. Built new search functionality into the next generation StratoDB scalable database management system.

President, XYZ University Young Women in Drug Prevention Essays, Tech. Facilitated monthly meetings to enhance a diverse community in software engineering. How To Get Motivated! Managed activities like monthly tech talks, workshops, and student panels. Here's a great list of Prevention Essays, GitHub projects beginners can chip in on a paper, to build a little instant PR. And this Quora thread gives suggestions for digi yellow man beginner projects that add quick experience to any resume.

Pro Tip: Don't lie. If you brag about a skill or experience in your resume, then can't demonstrate it in the interview, you're toast. Talk up only those strengths you can prove. Is Your Education Section Underperforming? It Might Be. Unless you went to Harvard or got a 4.0, your education section doesn't matter. That's absolutely false. Your education section doesn't matter if you make it generic. The education section of your computer engineering resume can be the thing that gets you hired.

Start off with the basics: The magic happens when you add your list of college wins. How To! Just like with work experience, make a master list of your achievements first. Then, pick those 4-6 items that best fit the job description. Can you spot the difference between these two software developer resume samples? Two Java Developer Resume Examples. 2 012-2015 BA, Software Engineering.

University of Washington Bothell. Received a BA in Software Engineering Took classes in C#, C++, Java, PHP, SQL, Perl, Python, Ruby. GPA 3.7 Worked in the cafeteria. As generic as a no-name box of Drug Programs, noodles. How To To Write A Paper! It could be anyone. Now clap your eyes on a software developer resume example that grabs the recruiter by the eyeballs: 2012-2015 BA, Software Engineering. University of Washington Bothell. Pursued my passion for Java server programming through six advanced classes. VP of american vs european, Jugger-Is, the campus machine learning club, 2014-2015. How To Get Motivated! Delivered an actionable webinar on X's The Ballot Bullet Essay, best practices for get motivated security in cloud servers that was linked to by the popular Dr.

Dobbs Journal. Beauty Vs European Beauty! My article, How Cloud Computing Will Change Your Life was published in the campus magazine. That second software engineering resume example stands out like red-font annotation. The point here isn't that you should go back in time and do all those the how to, activities. Uss Maine Sink! It's that you probably already have an impressive list and you just aren't using it. See the computer science resume template at the top of to write, this article for blood wedding sparknotes an example. Pro Tip: Need help tailoring your software developer resume to how to, the job description? See this great guide on how to Prevention Essays, tailor a resume any job offer. How to Put Skills on a Resume for how to get motivated a Software Engineer. Picture you're a hiring manager. You've got a folder with 250+ junior software engineer resumes.

Do you read every one? Like Tony Stark hunting a needle in a haystack, you use a magnet. In this case, that's an Applicant Tracking System (ATS). It sends the american beauty beauty, 75 resumes with the get motivated a paper, right keywords to another folder, which you read. Sink! The others go to folder #2. Don't worry. I'm going to show you how to pick the right software engineer resume skills keywords to get you hired. First, build your master list of get motivated a paper, every skill you have. Blood Wedding Sparknotes! Soft skills, hard skills, any skills at all. You'll refer to how to get motivated to write, it for every software developer resume you make, so spend a little time on it.

Start with the Scientific Method, sample resume keywords list below, then brainstorm your own. How To Get Motivated To Write A Paper! Look at other job offers online for inspiration too. Next, read the job offer. From a LinkedIn job description, we pulled the following skills keywords: Problem analysis, problem solving, distributed systems knowledge, Java, Java debugging, troubleshooting, open source knowledge, interpersonal, self-motivated. Last, match the skills you truly have with the digi yellow man, skills list from the job description. How To A Paper! But don't just list them. Prove them on your software developer resume by weaving them into your experience and education sections. Example of Skills on a Software Engineer Resume. Here's how to put skills on a software engineer resume.

Let's say you're applying for a job at digi yellow man, Airbnb. How To Get Motivated! It values teamwork, building full stack applications, creative thinking, data storage skills, and Java. So, include those in your skills section, experience section, and education section, like this: Skilled in digi yellow man, teamwork, leadership, creative thinking. Adept at data structures, problem solving, end-to-end coding.

Passion for get motivated to write a paper Java, SQL. Collaborated with multiple teams daily to blood, build full stack applications for dozens of clients. Used Java and SQL to build data storage solutions for several high-volume businesses. Spearheaded the company idea generation program. Improved efficiency in software development life cycle by 38%. Heavy concentration in data structures, Java, SQL. Organized the university hackathon in get motivated to write a paper, two consecutive years. Built a mock customer service webapp for a Senior Scholar project. See how the Scientific Method Essay, software engineer resume examples above paint a picture of a candidate who fills the bill like Larry Page or Bjarne Stroustrup?

Finally, there's no need to get motivated, fudge your skills. Blood Wedding Sparknotes! Find honest ways to show you've got the right stuff for the job. Otherwise you may get tripped up in the interview. Pro Tip: Once build a master skills list, you can start your own collection of software engineer resume templates that fit different skill sets. To make your computer engineering resume skills section crackle, follow the resume template at get motivated a paper, the top of this article. Prevention Essays! Also, see this guide: +30 Best Examples of What Skills to Put on to write a paper, a Resume (Proven Tips) How to Malcom X's The Essay, Add Other Sections for an Effective Resume.

Got awards, accolades, or certifications and want to put them in get motivated to write a paper, your resume? Add another section. Call it projects, awards, accolades, hobbies, or whatever seems to fit. Add only those things that'll make a hiring manager whistle like R2D2. Look at uss maine, the examples below from how to get motivated to write two distinct software engineer resumes: Moderated a security panel at Bullet examples, the Developer Week convention in a paper, 2014.

Shortlisted for a National Center for Women and digi yellow man, IT Award in 2015. Member, IAENG Society of Software Engineers since 2013. Attended the Software Engineering Professionals Hackathon in to write a paper, 2015. Rebuilt a Briggs Stratton lawnmower engine. Wedding! Walked dogs at a local shelter three times. Long walks on the beach. The activities in the first example will make a recruiter say, She's passionate.

I bet she'll bring that passion to our company. If you make a good enough resume, recruiters will weed-whack a path to how to, your front steps. In fact, as G.I. Joe might say, making a great software engineer resume is only half the battle. The other half is using it to point to beauty beauty, good examples of your work. That's why you need a solid online portfolio. Pro Tip: Use your online portfolio to how to, show off all your projects, then link specifically to the ones that make the most sense for american beauty vs european the job offer at get motivated, hand. Here's the Most Common Myth About Cover Letters. Nobody writes cover letters anymore, right? That's true. Of The Bullet Examples! If you're talking about the ones who don't get hired.

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Tom Gerencer is a founder and former owner of MediaNortheast Video Production and Training Without Boredom. A full-time writer in Scientific Method Essay, the fields of personal finance and career advice, Tom lives in West Virginia with his wife Kathy, two children and a couple of ornery dogs.

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Against Human Cloning Essays and Research Papers. Get Motivated A Paper. Cloning in general has been a rising debate across the globe since before Dolly the sheep was cloned in american beauty, 1996. The success of being able to . clone an animal brought scientists to wonder about a more challenging task, cloning humans . This consideration is morally wrong and should not be stood for. Some people seem not to realize the negative aspects that cloning would bring into a world which is already suffering. Religious standpoints, the growth of the population, and how to get motivated to write each human's individuality are.

Cloning , Hominidae , Human 985 Words | 3 Pages. is a mammal, her successful cloning seemed to open the Pandoras Box of the potential for Prevention Programs, human cloning , especially . when a scientific breakthrough occurred in 2004 when a team of South Korean and American scientists successfully cloned some human tissue. How To. We, as people are much divided on the issue of human cloning . Because science has advanced so quickly, we all have very mixed views about this cloning process, and the question still remains, Should we clone humans ? You cant play God! Some of. Wedding. Bone marrow , Cloning , Dolly 1085 Words | 3 Pages. In the past few years, human cloning has gone from how to a paper, a laboratory fantasy to a global debate. There are many arguments supporting . both negative and positive effects of human cloning . Human cloning raises a lot of challenging questions about human liberty, dignity, and identity. Will human cloning be a great step for man, or will it lead to moral abyss?

This question is asked all the time. With great research one would realize that with the implementation of human cloning , there would be a huge medical. Cloning , Hominidae , Human 2096 Words | 6 Pages. Hitler Clones and playing G-d? Hitler Cloning and scientists playing God are statements used to did the uss maine, describe human . cloning . When it comes down to the truth of the matter, human cloning is a scientific breakthrough that has a higher chance of helping society than hurting it. There are many people who are skeptical, believing that a world full of genetically reproduced miniature Hitlers will be created through human cloning . They imagine a second Holocaust starting as the cloned Nazi. Christianity , Cloning , Embryo 1274 Words | 4 Pages. Human Cloning Toni Rasmussen PHI 103 Informal Logic Stephen Carter September 20, 2010 Abstract: In this day and age . cloning seems to get motivated to write a paper, be one of the digi yellow man, most debatable concerns.

Scientists seem to get incredibly wound up thinking about all the potential that human cloning can present. Scientists also believe it is only a matter of time before humans are productively cloned. Some people, to to write a paper, some extent, are uncomfortable about human cloning and typically people tend to agree that cloning is morally. Cell , Cloning , Dolly 2074 Words | 6 Pages. Controversial Issues Paper Topic: Human Cloning Issue # 5 John A. Robertson, Human Cloning and . the digi yellow man, Challenge of Regulation, The New England Journal of Medicine, vol. 339, no. 2 (July 9, 1998), pp.

119-122. George J. Annas, Why We Should Ban Human Cloning , The New England Journal of Medicine, vol. 339, no. 2 (July 9, 1998), pp. 118-125. 10-16-00 In the article that I chose there are two opposing viewpoints on the issue of Should Human Cloning Ever Be Permitted? John A. Cloning , Embryo , Genetic disorder 1857 Words | 6 Pages. Cloning on Humans Over my years here at to write a paper college, I have learned a lot about humans and how our bodies . function. Scientific Method Essay. One of the classes that really stuck out to me and caught my interest was genetics. To Write A Paper. I enjoyed learning about our DNA and how it worked in Drug Prevention Programs Essays, terms of reproduction. A topic that we covered during the course of to write a paper, this class was on of the examples, cloning . I learned about the different types of a paper, cloning and when the focus on our in class discussions was on when did the uss maine sink, reproductive and therapeutic cloning , I. Cell , Cloning , Embryonic stem cell 1736 Words | 5 Pages.

Human Cloning Doctor Jerome Kassirer once said, Im willing to hold off completely on get motivated a paper, any . kind of human cloning , but Im not willing to hold off on research that could yield enormous therapeutic benefit. Human cloning is a controversial topic in science and medicine. While some are all for human cloning , others disregard the idea all together because they see the cons before they can understand the major advantages of pros. But what exactly is human cloning . Biotechnology , Cell , Cloning 1842 Words | 5 Pages. Human Cloning A Review on blood sparknotes, its effects Sreekanth Adepu Hari babu Annem English Prof. Cynthia Day November 24, . 2009 | | |Over the past 8 years since the a paper, development of first human clone human cloning is a controversial issue. According to Douglas coupland Cloning is great.

If God made the | |original, then making. Cell , Chromosome , Cloning 1287 Words | 6 Pages. Blood Sparknotes. Speech Title: Arguments for Human Cloning Specific Purpose: The purpose of this speech is to inform my audience about the . Get Motivated To Write. arguments given by supporters of human cloning . Thesis: Those who support human cloning make several claims of fact, value and policy to argue their case for human cloning . I. Introduction II. The majority of the population fears cloning only because they are uneducated and believe the digi yellow man, misconceptions spread by the popular media. a. Cloning is easier than most people. Bone , Bone marrow , Cancer 608 Words | 3 Pages.

Human Cloning (Excerpts from Human Cloning : Right or Wrong? by how to get motivated, Eryl Davies) The Raelian sect . was founded in France in 1973 by a journalist, Claude Vorilhon (Rael.) Active in eight-four countries, the sect claims over 60,000 members. The Raelian sect formed a cloning company called: Clonaid. For a heterosexual couple, perhaps it is the death of their child or a desire to take precautionary measures in Scientific Method, case their child dies young or unexpectedly. The precaution would be to preserve samples. Cell , Chromosome , Cloning 1406 Words | 5 Pages. of the American society sees human cloning as only science fiction, but what they dont know is how close to to write a paper, reality it actually . is. Uss Maine. With the technology that is available today, it does not seem crazy that human cloning could happen within my own lifetime. Cloning could have a very beneficial effect to our world, possibly one that we cant even fathom because the idea of human cloning is so complicated. After completing my research, I have concluded that human cloning should not continue to be researched. Biotechnology , Cell , Cellular differentiation 1702 Words | 6 Pages.

Human Cloning Human Cloning comes with two dangerous processes, reproductive cloning . (the creating of a new organism) and the therapeutic cloning (the creation of a new tissues or other biological products) which affects the ethics of human society. How To Get Motivated To Write. Scientists perceive cloning benefits all men and women, while religious leaders stress the digi yellow man, idea of cloning to how to get motivated a paper, be an unethical process. Although human cloning serves as an aid to the children and parents with conflicts, cloning is Scientific Essay completely unacceptable. Cloning , Human , Human cloning 1039 Words | 3 Pages. The Legal Future of Human Cloning. The Future of Human Cloning in America: A Legal Perspective On July 6, 1996, a historical bookmark in modern science was . placed as scientists successfully cloned Dolly the Sheep. Dolly was the first mammal to ever be cloned, and from her existence grew several very real concerns, most notably the possibility of humans also being cloned in a laboratory. This new possibility brought up the ethical implications of cloning a human being and President Bill Clinton quickly passed an executive memorandum. How To A Paper. Cloning , Human cloning , President of the Method Essay, United States 1691 Words | 5 Pages.

?DEFINITION Human cloning is the process of get motivated to write a paper, creating a genetically identical copy of a human / human . Digi Yellow Man. part. CONS (anadid o cambiad cosas si queries/ podeis) Unpredictable outcomes The greatest risk presented by human cloning is the unpredictability of the development of intelligence in the clones. There is a possibility of faster aging - Because an older cell is often being used to create a human clone, this could result in how to to write, premature aging issues or premature death. There is a reduced sense. Ageing , Cloning , Death 705 Words | 3 Pages. Biology Should human cloning be allowed? Questions started with a sheep named Dolly.

Dolly was a clone sheep born on February . 12th 1997. Ever since Dolly has existed scientist across the globe have been studying cloning and we are now presents with the question, should human cloning be allowed? In order to debate the potential of human cloning , we must first consider how it ties in to certain aspects of human society. Blood. In society today, public opinion about how to get motivated to write a paper human cloning is divided. The supporters. Cell , Cloning , Embryo 1253 Words | 4 Pages. Method Essay. The Debate on Human Cloning In the past decade, there has been no slackening of the how to to write a paper, pace of scientific research and discovery. Method Essay. . On a daily basis, scholarly publications and to write a paper mass media divulge new and profound discoveries that seem to open doors that no one knew existed. Drug. Through their research, scientists have relentlessly attempted, time and time again, to penetrate into the very core of the universe and to to write, unveil the essence of what constitute human beings. Few breakthroughs in research epitomize. Bioethics , Cloning , Embryo 1472 Words | 4 Pages. Legalizing Human Cloning Laura Johnson Philosophy 103 May 10, 2010 Part I: Thesis We are in Drug Prevention Essays, a technological and to write . medically advanced age to where anything is possible.

Technology and medical advances combined us as a people have the ability to gain from each opportunity that we invent. For example, we now have the capability at our fingertips to connect with friends and family through the World Wide Web, this in Scientific Method Essay, which is given to us by way of technological advances. Through medical. Cloning , Dolly , Domestic sheep 1547 Words | 5 Pages. Human Cloning: Is It Dangerous to Human Beings? Cloning is the method that produce an exactly identical genetic of another one. It has been studied and researched for more than ten years with . vegetables and animals. The first success with living thing was a sheep named Dolly and that was one of the experiments that show how far human can get to the high stage of how to to write a paper, scientific technologies.

Since then, the idea of american beauty vs european beauty, human cloning was conceived. Some people think that human cloning should be permitted because it can brings great benefits to mankind. Adolf Hitler , Barack Obama , Cloning 1043 Words | 3 Pages. Human Cloning A Moral Issue Alex Correa Ms. Coveney ENG 110 September 21, 2011 Essay 2 The topic of . human cloning has become one of the most debated topics in the world since 1997 when Dr. Ian Wilmot revealed he had successfully cloned an adult sheep named Dolly. Since the cloning of to write, Dolly, there has been ongoing discussions regarding the morally right and morally wrong of human cloning . According to the Gallup Polls, by Gallup, Inc., as of May, 2010 63 percent of. Cloning , Dolly , Embryo 719 Words | 3 Pages. Human Cloning , Is it Ethical Human cloning is and has been the subject of moral debate in the . United States and around the Prevention Essays, world, and because of this it has raised many ethical questions. One thing that I do have in common with our previous president Bush is his principle that it is unethical to create human life for destroying it.

I never thought about to write human cloning and its relevance until I watched the movie, called The Island. The Island is a sci-fi action thriller that reportedly cost about. Cell , Chromosome , Cloning 1440 Words | 4 Pages. Cloning Storm Cloning is one of the controversial topics in this day and Scientific Essay age, and with the media and technology . implanting the idea of how to get motivated to write a paper, cloning in our psyche by using it in various films and fiction novels some people started to develop right and left views on this purely scientific topic as some believe that cloning is destructive and will lead to american beauty vs european, bad consequences to our nature as humans while other people believe that cloning is something that we will likely benefit from and can help. Cloning , Human , Human cloning 1063 Words | 3 Pages. What Are the how to to write a paper, Ethical Issues of Human Cloning. regarding the issue of human cloning in countries such as the United States, the United Kingdom and Drug Programs Australia which have made . attempts or have done research in reproductive cloning . How To Get Motivated. Countries like Australia have prohibited human cloning in 2006. (NHMRC, 2007) Advocates who involve congress members, editorial writers, fertility specialists. and beauty vs european beauty so on how to a paper, gave benefits of human cloning , yet not enough to justify the digi yellow man, moral and ethical issues underlying the how to get motivated, controversy. Human cloning refers to the creation. Bioethics , Cloning , Dignity 1463 Words | 5 Pages. Malcom X's The Of The Bullet Essay. Human cloning is and has been the subject of moral debate in how to to write, the United States and around the world, and because of this it has . raised many ethical questions. Such as, should it be done?

Could the clone survive? If human cloning technology were safe and widely available, what use would they have for it and what reasons would they have to uss maine sink, use it? If scientists were capable of cloning without failure, what would the welfare of the clones be like? Would the clone be its own person or would it be an. Cell , Cloning , Dolly 2408 Words | 6 Pages. The Evils of a paper, Human Cloning About a decade ago, Scientist at Roslin Institute from Scotland surprised the entire world when the american vs european, . announced that they have cloned an adult sheep on how to get motivated a paper, February 22, 1997, Dolly, (Dudley 9). Scientists shocked the world because many believed that it was impossible to produce a clone from a donor cell taken from an adult rather than an, embryo, many resulting embryos had died before (Dudley 11).

Dolly was created because, scientist at the Roslin Institute wanted to did the uss maine, research. Childbirth , Cloning , DNA 2230 Words | 6 Pages. Human Cloning : Fact or Fiction? Good or bad? Jeremy Parks 08/05/13 Microbiology Unit 6 Assignment 1 July 5th, 1996, Dolly . the Sheep was born. Dolly was the first animal to be cloned that survived as a healthy organism. This event opened a proverbial can of worms . How To. The possibility of Malcom Ballot of the Bullet Essay examples, cloning and the excitement of it all was evident in movies of the time. Jurassic Park, Mulitplicity and Invasion of the Body Snatchers to just name a few. Dolly was a great scientific achievement and set the.

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Biotechnology is a field of beauty vs european, biology that involves the use of living things . in to write, engineering, technology, medicine and other useful applications. The concept of Drug Prevention Programs, this field includes a range of get motivated to write, procedures for modifying living organisms according to Scientific Method, human purposes. A Paper. One major subject in biotechnology is human cloning . This is the Drug Essays, asexual creation of get motivated, a genetically identical copy of blood wedding, a human , human cell or a human tissue. It is a controversial issue. Cell , Cloning , Embryo 1151 Words | 4 Pages. Lam Dang Reading and get motivated to write a paper Writing 108 June 24, 2012 Outline: Banning Human Cloning I. Introduction ? Succeeded on . Ballot Of The. animal, Dolly sheep = on human = treat diseases, especially infertile couples. ? However, I oppose to how to a paper, clone on human because: 0 Uncontrolled results 0 Expensive costs: to do research and treat 0 Ethical/ moral concerns II. Support 1. Uncontrolled results: ? How long to Essay, live = short life (Ex: Dolly only can live 6-a half years).

Cloning , Dolly , Domestic sheep 862 Words | 4 Pages. Cloning Humans for Organs: Introduction: On June 26, 2000, scientists involved in the Human Genome Project . How To Get Motivated To Write A Paper. announced their success in Bullet, mapping the human genome. This has created an enormous controversy over property rights in human tissue. With advances in biotechnology and the advent in cloning , it seems likely that these issues will continue to cause controversy in the years to come. As the shortage of get motivated a paper, organs available for transplantation continues to grow, new methods of obtaining organs and. Biotechnology , Cell , Cloning 1937 Words | 6 Pages. Human Cloning: the Drug Essays, Negative Effects on Society. Human Cloning : The Negative Effects on Society Human cloning is possible but also dangerous to . society.

One day during an assembly in how to get motivated to write, March 1977, a heated debate began over the topic of human cloning . Both sides were getting very involved in the argument. Did The Uss Maine Sink. Suddenly a group of protestors stormed the stage. Get Motivated A Paper. They were protesting human cloning while chanting, We shall not be cloned! During the protest the group held up a sign that said: We will create the perfect race ? Adolf Hitler (Lester and. Clonaid , Cloning , Dolly 1576 Words | 4 Pages. Reconstructive Human Cloning, Ethical Issue. Is Reconstructive Human Cloning Unethical? Since the Ballot Bullet Essay examples, beginning of the mid-20th century people were trying to progress the how to get motivated to write a paper, . human era and thus begin a new era of humanity. Many of these theoretical approaches and beauty trials have failed due to lack of experience and get motivated the technology. One of the first ones to experiment with an Method, ultra- humans or super humans were Germans during the Second World War under the command of how to, Adolf Hitler. Experiments on twin children in concentration camps were created to Method Essay, show the. Cloning , Health , Human 1453 Words | 4 Pages.

The Ethics of Human Cloning In the beginning, God created Earth, thus creating man and woman. Or at how to get motivated least that is what some . believe. Programs Essays. However, over thousands of years, history, culture, and technology has slowly laid out the foundation of life; the human age and civilization went through a metamorphosis. Now, in modern day and age, technological advances persist daily, and the world does not stop evolving. How To. We, as people, continue to learn more about ourselves, about our planet, about the stars. Cloning , Human , Human cloning 1071 Words | 3 Pages. SAR: Human Cloning Is It a Viable Option? The topic of cloning has grown in american beauty vs european beauty, recent years due to the steady . increase in technology and innovation. Ms. Angela A. Boatwright writes a very interesting and informative essay regarding human cloning . After reading her article, I recognize that my personal viewpoint on how to a paper, this subject is fairly close to what Boatwright has clearly communicated in her paper. The concept of cloning and the experiments involved should never include human life as a subject.

Cloning , Hominidae , Human 973 Words | 3 Pages. Ethics of human cloning has become an outstanding issue in Scientific Essay, the past few years. Even though both sides agree that . cloning technology is one of the how to to write a paper, greatest achievements, they still have a big gap in Malcom X's The Ballot, accepting its ethnicity. On one hand, a number of people have extremely appreciated human cloning because this might be the last chance or even the get motivated to write, only way for them to blood wedding, preserve the how to to write a paper, priceless images from their lovers. Method Essay. On the other hand, many others have claimed that cloning human beings would seriously. To Write A Paper. Ethics , Hominidae , Human 1633 Words | 3 Pages. Under what conditions (if any), should human reproductive cloning be permitted? Kubiak, J.Z. and Johnson, M.H. (2001). . Human Infertility, Reproductive Cloning and Nuclear Transfer: a Confusion of Meanings. Bioessays, 23(4), 359-64.

This article argues that confusions in the semantic meanings of human reproductive cloning may hinder future scientific progressions to discover an ethically viable solution to infertility treatments. Kubiak and Johnson postulates that the term nuclear transfer. Clone , Cloning , Dolly 1669 Words | 5 Pages. Is Human Cloning Acceptable in Today's Society? Is Human Cloning Acceptable in Todays Society ? INTRODUCTION In my paper I will be talking about cloning , how . scientists are trying to clone humans and if it acceptable in Drug Essays, todays society and should it be. I will also be informing the reader about how scientists try to do it and why. Cloning is the get motivated to write a paper, process of producing populations of genetically-identical individuals that occurs in nature when organisms such as bacteria, insects or plants reproduce asexually (amphibians are the did the, best examples) . Cloning , Dolly , Domestic sheep 1228 Words | 4 Pages. Cloning (advantages and disadvantages) On our planet, today everything is possible and . humans made it that way.

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Cloning , Dolly , Embryo 2373 Words | 7 Pages. Starting in 1997, when the very first mammal ever cloned-a sheep named Dolly-there have been many debates over cloning and beauty vs european genetic mapping. . How To Get Motivated To Write A Paper. The act of sparknotes, cloning and a paper genetic mapping that occurs both in Drug Essays, humans and in animals is morally wrong as both researchers and non-researchers tend to take advantage of these very touchy situations. How To To Write. There are many risks involved in reproductive cloning such as; failure resulting in death or mutations, people playing god by adjusting the way there babies look or. Cloning , DNA , Dolly 1611 Words | 4 Pages. The benefits of human cloning to our society. Benefits of Cloning to our Economy and Society Have you wished that there were two of you? This question is digi yellow man one that everyone has asked himself . or herself that some point in their lives. a group of Scottish scientist had this in mind back in how to get motivated to write, the tail end of the nineties when they cloned the first living clone in existence. That clone was named Dolly the sheep; dollys existence was topic of such debate on both the scientific and religious aspects of our society. To the point that human cloning was outlawed. Bill Clinton , Cloning , Dolly 1237 Words | 5 Pages. definitely be bizarre watching yourself grow up all over again.

In order to obtain that copy of Drug Programs Essays, yourself, human reproductive . cloning would have to take place. Human reproductive cloning is the process of taking genetic material from a person, and using it to make an embryo, in to write, the hopes that it will develop properly, and in the end become a child. The beginnings of beauty, what we today refer to as cloning , actually go back to the early part of the twentieth century?1901 to be exact. Get Motivated A Paper. Hans Spemann (1869-1941). Abortion , Cloning , Dolly 1778 Words | 5 Pages. Cloning - is the technique of making an identical copy of digi yellow man, something. Get Motivated To Write. DNA () - stands for did the uss maine, deoxyribonucleic acid, is defined as a nucleic . acid that contains the genetic code. How To Get Motivated To Write. Cell () is the smallest part of a man, animal or plant that is able to function independently. Stem cell ( ) is a type of cell that can produce other cells which are able to develop into any kind of cell in the body. Beauty. Embryonic cell 4 Cloning In biology, cloning is the how to, process of producing similar.

Cell , Cloning , DNA 854 Words | 3 Pages. 2003 Disadvantages of Human Cloning - Loss of Human Identity Cloning has always been a subject . whose thoughts both fascinates and frightens the world. On February 27, 1997, a stunning announcement appeared in digi yellow man, the British journal Nature that rocked the scientific world: for the first time ever recorded, a mammal- a lamb named Dolly had been successfully cloned from an adult cell. Coinciding with this shocking proclamation, scientist reported the successful cloning of a rhesus monkey, a primate. Bioethics , Cloning , Dolly 1977 Words | 6 Pages. Cloning Technology Cloning technology is defined as the creation of an organism that is genetic copy from another this is . mean that every single bit of DNA is the same between the two, the Biotechnological DNA cloning combine the technology to produce multiple copies will producing a group of genetically identical cells or clone and all from single ancestor. Cloning process of reproduction in plant and micro cells are called reproductive organism, this is now as vegetative reproduction because.

Agriculture , Cell , Cloning 1514 Words | 4 Pages. Risk and Challenges of Human Cloning . Now that a dog and how to a sheep has been cloned, can human . Digi Yellow Man. cloning be that far behind? How is cloning important to how to get motivated a paper, future therapeutic medical advances, such as safe organ transplant? Human cloning research holds the answer to these questions. But still, the blood wedding, development of human cloning are slow and tough as it faces great challenges from the society that concern about moral and ethical abuse that the fallout of human cloning may produce.

According. How To To Write. Bioethics , Cloning , Ethics 1415 Words | 5 Pages. Positives and Negatives of Cloning. Pros and Cons of Cloning The process of cloning has remained one of the most controversial topics as debates continue about the . pros and cons of Cloning . Cloning which is the process of duplicating DNA or living stem cells can be dated back to digi yellow man, over 200 years ago. The two most common types of cloning are Therapeutic Cloning and Reproductive Cloning . Therapeutic cloning is a process that specifies the use of cloning stem cells for the treatment of an illness or disability (Ham, 2007). Reproductive.

Cell , Cloning , Human 1304 Words | 4 Pages. The Moral Implications of Cloning. Outside the lab where the cloning had actually taken place, most of how to get motivated, us thought it could never happen. Oh we would say that perhaps at beauty beauty some . point in the distant future, cloning might become feasible through the get motivated a paper, use of sophisticated biotechnologies far beyond those available to beauty, us now. But what we really believed, deep in our hearts, was that this one biological feat we could never master. Dr.

Lee M. How To A Paper. Silver, 1997. Digi Yellow Man. On February 23, 1997, Doctor Ian Wilmut successfully cloned the world's first mammal. Get Motivated To Write A Paper. Cloning , Human , Human cloning 2001 Words | 6 Pages. Vs European. The advantages and disadvantages of cloning. process of cloning . It is how to get motivated simply a form of asexual reproduction.

Cloning is a big issue in todays world of Drug Prevention, science because of the . Get Motivated. potential benefits, the risks involved, and medical, ethical and religious conflicts. Plants and blood animals have already been cloned and stem cell research is to write already going ahead, and maybe one day science will be able to american, clone a human . The division of how to get motivated a paper, opinion on cloning will require many forums of Drug Prevention Essays, discussion to cover all aspects. There are serval types of cloning , Reproductive. Cell , Cloning , DNA 1613 Words | 5 Pages. How To Get Motivated To Write. Cloning Human Beings: An Assessment of the Ethical Issues. Cloning Human Beings: An Assessment of the Ethical Issues Pro and Con In Dan W. Brock's essay, Cloning . human beings: an assessment of the ethical issues pro and con, Brock tries to take a neutral stance on the subject and give both the positives and negatives of cloning human beings. While doing this he talks about the Essay, individual and societal repercussions, what it means to be an individual and to write human rights. He takes a strict secular approach to both sides of the argument and doesn't involve. Cloning , Ethics , Human 900 Words | 3 Pages. Of The. Preventing a Brave New World; Cloning of Human Embryos. Abstract Leon Kasss provides a compelling article on why the world should ban cloning . Kass article, Preventing a Brave New World, . How To Get Motivated A Paper. provides Kass theories of if society progresses on its current path, society would start to turn to cloning as an alternative to did the, typical reproduction.

Cloning offers a way to design an offspring rather than the typical experience of how to to write a paper, child raising. Kass believes if cloning is sink not banned, society will be more dependent upon technology and to write be at technologies. Cloning , Ethics , Human 944 Words | 3 Pages. What is the worlds view on cloning ? 1. Bob Sullivan, Religious little consensus on cloning ; 2006, October 4, 2007 Bluford . library Summery- This article is about some religious views of cloning . Sullivan explains, how the religious groups say that its ok in a way to clone. Analysis- Do I think cloning meddles with gods universe No, I believe we were in Scientific Method, this universe for how to, a reason; a reason to Scientific Method Essay, discover and explore. In the Muslim, Jew community both are not in favor of fertilizing embryos. Cloning , Dolly , Domestic sheep 1244 Words | 4 Pages. most people think of cloning , they think of how to a paper, a scientist that uses DNA to make an Scientific Method Essay, exact replica of someone . Cloning is a lot more . To Write. complicated and more controversial than that . According to the American Heritage Dictionary Cloning is defined as A group of cells, or organism that is when did the uss maine sink descended from and genetically identical to a common ancestor , such as a bacterial colony whose members arose from a single original cell . Ever since August,1,2001 Cloning had been banned . How To To Write. Cloning was banned for many.

Cloning , Disease , Dolly 2008 Words | 6 Pages. Malcom Ballot Bullet. Cloning Should Not be Legalized Human cloning has been a very controversial issue for a number of years. How To Get Motivated To Write A Paper. In . order to understand cloning better is important to Scientific Method, recognize what cloning is. A clone is an exact genetic replica of a DNA molecule, cell, tissue, organ, or entire plant or animal. This may be done through reproductive cloning or therapeutic cloning known as stem cell cloning . (Coalition for the Advancement of Medical Research) Human cloning raises the issues of whether or not a cloned. Cloning , DNA , Dolly 2444 Words | 7 Pages. Brave New World and get motivated to write a paper the Implications of american beauty, Cloning. Cloning is no longer a thing of science fiction- it is the present's most controversial issue. Cloning is complex and involves . many people and machines. First, a suitable embryo donor must be found.

Once a doctor has obtained the how to a paper, egg, he or she will give it to an expert in cloning who will develop the fetus, or underdeveloped baby from the DNA of a live animal, in a laboratory and finally clone it. Cloning , if legalized, would be used primarily for medical purposes. In particular, cloning humans . Aldous Huxley , Brave New World , Cell 1309 Words | 4 Pages. The negative points of cloning greatly outweigh the positive. Beauty Vs European. This is proved by the fact that cloning is currently illegal in . To Write. Australia, so other people must feel the same way. With plant cloning , the genetic makeup is the same for each clone made from the same DNA.

If the Malcom Ballot examples, original DNA has a disease, all of the how to, clones will have it too. Plant clones will also be vulnerable to the same diseases and environmental factors as the original plant. This means that if all the cloned plants are in one area. Cloning , Evolution , Gene 749 Words | 3 Pages. Starting with an explanation of what cloning is and the two main types of the technology: reproductive cloning and therapeutic . cloning , this paper goes on to explore the ramifications these technologies may have in different areas of our society with respect to government, funding issues for research, religious views, education for our children on cloning issues and lastly the publics response to cloned animal produce. Sociological Impacts of Cloning Cloning is the production of one or more individual.

Cloning , Embryo , Human 1913 Words | 5 Pages. Biotechnology in Cloning Brian Hopkins South University Online BIO1020 Unveiled to X's The of the Bullet Essay, the worlds press by Ian Wilmut and colleagues at the . Roslin Institute in Midlothian, Scotland on how to to write, February 22, 1997, Dolly the blood sparknotes, sheep was the first mammal to be successfully cloned from an adult cell. To Write. She was actually born a few months earlier on July 5, 1996. The world was certainly awed when the first superstar sheep Dolly was introduced. It was that date in history when the world first heard about Method Essay successful. Cloning , Dolly , Domestic sheep 1011 Words | 3 Pages. Should Cloning Be Banned Completely, Regulated, or Fully Legal? Should Cloning be banned completely, regulated, or fully legal?

When it comes to the topic of cloning , many writers embrace . How To To Write. different views on the use of this technology. Zabludoff the writer of Cloning : Is It Inevitable? is against Malcom X's The Ballot of the examples the practice of to write, cloning because according to vs european, him cloning would not entirely work, as there is no such thing as a cloned human , hence, the practice of cloning is pointless. Get Motivated To Write. On the other hand, Krauthammer also encompasses similar views as Zabludoff in his article Of.

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Essay on Industrial and Microbial Biotechnology. In this essay we will discuss about Industrial and Microbial Biotechnology. After reading this essay you will learn about: 1. Introduction to Industrial Biotechnology 2. Characteristics of Microbes used in Industrial Biotechnology 3. Get Motivated To Write A Paper. Choosing Micro-Organisms for Industrial Microbiology 4. Culture Media 5. Major Products of Industrial Biotechnology. Essay on the Introduction to Industrial Biotechnology Essay on the Characteristics of Microbes used in Industrial Biotechnology Essay on Choosing Micro-Organisms for Industrial Microbiology Essay on Culture Media Essay on Major Products of Industrial Biotechnology. Essay # 1. Introduction to Industrial Biotechnology: Industrial biotechnology, also known as white biotechnology, is the american beauty application of biotechnology for industrial purposes. How To To Write. It is Drug Prevention Programs Essays, also redefined as bioprocess technology.

When our early ancestors made alcoholic beverages, they used a bioprocess. The combination of yeast cells and get motivated to write a paper, nutrients (cereal grains) formed a fermentation system in which the organisms consumed the nutrients for their own growth and produced by-products (alcohol and carbon dioxide gas) that helped to make the Malcom X's The of the Bullet Essay examples bever­age. Bioprocess technology is an extension of ancient techniques for how to to write developing useful pro­ducts by digi yellow man taking advantage of natural biological activities such as production of enzymes (used, for example, in food processing and how to to write, waste management) and antibiotics. Use of living material offers several advantages over con­ventional chemical methods of production. They usually require lower temperature, pres­sure, and pH. They can use renewable re­sources as raw materials, and greater quanti­ties can be produced with less energy consump­tion. With the development of automated and computerized equipment, it is becoming much easier to accurately monitor reaction conditions and thus increases production efficiency. As advancements in Prevention, industrial technology, par­ticularly separation and purification tech­niques, are made, commercial firms will be able to economically produce these substances in how to to write, large amounts, and thus make them available for blood sparknotes use in medical research, food processing, agriculture, pharmaceutical development, waste management, and numerous other fields of science and industry. The very basis of In­dustrial biotechnology is a paper, microbiology.

All the various production strategies bioprocess tech­nology solely depends on Malcom Bullet Essay, various species of mi­crobes. Essay # 2. Characteristics of Microbes used in Industrial Biotechnology: Microorganisms which are used for industrial production must meet certain requirements including those to be discussed below: 1. The organism must be able to grow in a simple medium and get motivated to write a paper, should preferably not require growth factors (i.e., pre-formed vi­tamins, nucleotides, and acids) outside those which may be present in the indus­trial medium in which it is grown. 2. The organism should be able to blood sparknotes grow vigo­rously and rapidly in the medium in use. A slow growing organism no matter how efficient it is, in get motivated to write, terms of the production of the target material, could be a liability. 3. Not only should the when uss maine organism grow rap­idly, but it should also produce the desired materials, whether they be cells or meta­bolic products, in as short a time as pos­sible, for to write a paper reasons given above. 4. Its end products should not include toxic and other undesirable materials especially if these end products are for Method Essay internal con­sumption. 5. The organism should have a reasonable ge­netic, and hence physiological stability.

6. The organism should lend itself to a suit­able method of product harvest at get motivated a paper the end of the fermentation. 7. Wherever possible, organisms which have physiological requirements and protect them against competition from contami­nants should be used. 8. The organism should be reasonably resis­tant to predators such as Bdellovibrio spp or bacteriophages. 9. Where practicable the Method organism should not be too highly demanding of oxygen as aera­tion (through greater power demand for agitation of the fermentor impellers, forced air injection, etc.) contributes about 20% of the cost of the finished product. 10. Lastly, the organism should be fairly eas­ily amenable to genetic manipulation to en­able the establishment of strains with more acceptable properties. Essay # 3. Get Motivated To Write A Paper. Choosing Micro-Organisms for Industrial Microbiology: The first step of wedding sparknotes, industrial biotechnology is to how to get motivated a paper find out a suitable microorganism which will be mostly fulfilling above desired characteristics.

Microbes important to bioprocesses have been isolated from natural sources such as soil samples, water, and spoiled bread and fruit. Cultures from Method Essay various sources were examined in order to get motivated to write a paper identify strains with desirable char­acteristics. Only a minor portion of the micro­bial species in most environments has been isolated or cultured. Due to this there is Essay, a con­tinuing effort throughout the a paper world to find new micro-organisms which will be useful to indus­trial biotechnology. Followings are the techniques used for the development of new noble strains of micro­organisms:

1. By the Process of Genetic Manipulation : The fundamental idea behind this technique is to change the when sink genetic makeup of the microbe in order to develop new genetic traits which will be profitable to us. This can be achieved by following ways: (a) Protoplast Fusion: Protoplast fusions now widely used for the improvement of fungal organisms like yeasts and get motivated to write a paper, moulds. To achieve protoplast fusion protoplasts of these microbes are obtained by growing the cells in an isotonic solution followed by treating them with enzymes such as cellulose and beta-galacturonidase. This step is followed by the regeneration of Prevention Programs, proto­plasts by using sucrose. The fused proto­plasts result in the formation of hybrids which can be identified by means of selec­tive plating techniques. Mutations, especially the one which are achieved with the how to get motivated to write help of chemi­cal mutagens and ultraviolet light, have been very useful in developing new strains of microbes. For example, ordinary cul­tures of digi yellow man, Penicilliumnotatum yields low concentrations of penicillin. Later on a strain of Penicilliumchrysogenum (NRRL 1951) was isolated by repetitive selection procedure which was further improved through the process of mutation.

This im­proved strain of Penicilliumchrysogenum gives 55-fold higher penicillin yields than the original Penicilliumnotatum cultures. (c) Site directed Mutagenesis: In the pro­cess of site directed mutagenesis short lengths of chemically synthesized DNA se­quences, having the genetic information of novel traits, can be inserted into the ge­nome of the target microorganisms. How To Get Motivated To Write. This process results in the genetic alterations of the microbe that leads to a change of one or several amino acids in the target protein. As a consequence of this there are many changes in protein characteristics, and results in new products. These ap­proaches are part of the field of protein engineering. 2. By the Production of Transgenic Microbes : New varieties of microbes can be developed by encouraging the transfer of nucleic acids be­tween them. This process involves the trans­fer of genes, having the genetic information for Method the synthesis of a specific product, from one organism into another. The recipient organ­ism becomes a transgenic organism. Example of such a microbe is Superbug developed by A. M. Chakarabarty in 1974. This transgenic microbe has the capability of hydrocarbon deg­radation.

For the production of superbug he used the microbe Pseudomonas putida. It is capable of utilizing complex chemical com­pounds like hydrocarbons. It is also called oil eating bug. Petro-products, including oils, generally contains octane, xylene, camphor and naphthalene. No single strain of P. putida is able to digest all these four petrochemical.

Dr. How To Get Motivated A Paper. Chakarabarty introduced four plasmid DNAs from four different strains into Scientific Essay one single cell of Pseudomonas putida and get motivated a paper, generate a superbug. Recombinant DNA techniques can also be used for the generation of transgenic microbes. This technique has been widely exploited for the production of vaccines; for example the vac­cine for the foot and vs european beauty, mouth disease of livestock. Genetic information for a foot-and-mouth dis­ease virus antigen can be inserted into E. coli. This is followed by the expression of get motivated, this ge­netic information and synthesis of the gene product for blood sparknotes use in vaccine production. 3. By Modifying Gene Expression: In addition to inserting new genes in organ­isms, it is also possible to filter the pattern of gene regulation. This is possible by means of changing gene transcription, fusing proteins, creating hybrid promoters, and by removing feedback regulation controls. 4. Preservation of Selected Strains of how to get motivated, Micro-organisms : Once we have selected the microorganism or virus catering to the industrial interest, then it must be preserved in Drug Programs, its original form for any further use and research. To Write. There are dif­ferent methods for microbial preservation.

Suitable methods are selected based on the: (a) Type of micro-organism, (b) Effect of the preservation method on Prevention Essays, the viability of micro-organism, (c) Frequency at which the cultures are with­drawn, (d) Size of the microbial population to how to get motivated a paper be pre­served, (e) Availability of resources, and. (f) Cost of the Drug Prevention Programs Essays preservation method. Followings are some methods: This involves removal of water from the cul­ture. Desiccation is used to preserve actinomycetes (a form of fungi-like bacteria) for get motivated to write a paper very long period of time. The microorganisms can be preserved by Malcom X's The Ballot of the desiccating on sand, silica gel, or paper strips. Microorganisms are grown on agar slopes in test tubes and stored at 5 to -20 C for six months. If the surface area for growth is cov­ered with mineral oil the micro-organisms can be stored for one year. This is the most commonly used technique to store micro-organisms for a long period. Stor­age takes place at temperatures of less than -196 C and even less in vapour phase.

Micro­organisms are made stationary and suspended in a cryoprotective agent before storing in li­quid nitrogen. This method is especially used for sporulating microorganisms (organisms that produce spores). They Eire sterilized, inoculated, and incubated to a paper allow microbial growth, then dried at room temperature. The resultant dry soil is stored at 4 to 5 C. This process is also known as freeze-drying. The microbial culture is Scientific Essay, first dried under vacuum, filled in ampoules (glass vessels) then frozen. This is a most convenient technique, since it is get motivated a paper, cheap to store and easy to Prevention Programs ship. The disadvantage is get motivated to write a paper, that it is X's The Essay, difficult to open the freeze dried ampoules; also, several subcul­tures have to be done to how to get motivated a paper restore the Drug Prevention Programs Essays original characteristics of the micro-organisms.

5. How To Get Motivated To Write. Growth of Prevention Essays, Micro-organisms : In the a paper industrial processes the selected micro-­organisms are grown using specifically de­signed culture media under very carefully con­trolled conditions such as temperature, aeration, etc. Media requirements depend on blood sparknotes, the type of microorganism being used in the fermentation process, but the basic requirements remain the same, which includes: (a) Source of energy. (b) Water, carbon source. (c) Nitrogen source. Designing the media for small scale labo­ratory purpose is relatively easy, but me­dia for industrial purpose are difficult to prepare. An ideal culture medium has the follow­ing features: (a) Allow high yield of the desired product and at fast rate.

(b) Allow low yield of undesired products. (c) Be sterilized easily. (d) Yield consistent products i.e., minimum batch variation. (e) Be cheap and readily available. (f) Be compatible with the fermentation pro­cess. (g) Not pose environmental problems before, during, or after the get motivated fermentation process. The culture medium will affect the design of the fermenter. For example, hydrocarbons in the media require high oxygen content, so an blood wedding sparknotes, air-lift fermenter should be used. Natural media ingredients are cheap but they have high batch variation. On the other hand pure ingredients (also called defined media or for­mulated media) have very little batch varia­tion but are expensive.

The media should sup­port the metabolic process of the micro-orga­nisms and allow bio-synthesis of the desired products. Carbon Energy source + Nitrogen source + Nutrients > Products(s) + Carbon Dioxide + Water + Heat + Biomass. Media are designed based on the above equation using minimum components required to produce maximum product yield. Important components of the medium are discussed as follows: 1. Carbon Sources: Product formation is directly dependent on the rate at which the carbon source is metabolized. Also the how to get motivated to write main product of fermentation deter­mines the type of Scientific Method Essay, carbon source to get motivated be used. Scientific. Carbon sources include carbohydrates, oils and fats, and hydrocarbons.

These are the most com­monly used carbon sources in the fermen­tation process. Starch is easily available carbohydrate obtained from to write maize, cere­als, and potatoes. It is widely used in alco­hol fermentation. Grains like maize are used directly in the form of Drug Essays, ground pow­der as carbohydrate. Malt and beer made from get motivated a paper barley grains contain high concentra­tions of different carbohydrates like starch, sucrose, cellulose and other sugars. Uss Maine Sink. Su­crose is obtained from get motivated to write a paper sugar cane and molasses. Molasses is one of the cheapest sources of blood, carbohydrate. It contains high sugar concentration and other components like nitrogenous substances and vitamins and is used in alcohol, SCP (Single-cell Protein), amino acid, and organic acid fer­mentations. Extraction and purification of the products is to write a paper, expensive. Sulfate waste liquor is the by-product of the paper in­dustry; it contains carbohydrates and is used in yeast cultivation. Whey is the by-­product of digi yellow man, dairy industry.

It is get motivated to write a paper, used in al­cohol, SCP, gum, vitamins, and lactic acid fermentation. Vegetable oils are used as a carbon source. Oils provide more energy per weight compared to sugars. Programs Essays. They also have anti-foaming properties but are ge­nerally used as additives rather than as the sole carbon source. Examples are ol­ive oil, cotton seed oil, soya bean oil, lin­seed oil, and lard (animal fat). C12-C18 alkanes can be used as carbon sources. They are cheap, and have more carbon and energy content per weight than sugars. They can be used in organic acids, amino acids, antibiotics, enzymes, and a paper, proteins fermentation. 2. Nitrogen Sources: Ammonia, ammonium salts, and urea are the most commonly used nitrogen sources in the fermentation process.

Ammonia also serves the purpose of pH control. Other substances used as nitrogen sources are corn-steep liquor, soya meal, peanut meal, cotton seed meal, amino acids, and proteins. Calcium, chlorine, magnesium, phosphorous, potassium and sulfur are the essential miner­als for all media. Other minerals like copper, cobalt, iron, manganese, molybdenum, and zinc are needed in trace amounts and are gen­erally present as impurities in other compo­nents. The specific concentration on these ele­ments depends on the micro-organism being used.

4. Growth Factors: Vitamins, amino acids, and fatty acids are used as growth factors in the fermentation process to complement the Scientific cell components of the mi­cro-organisms. 5. Chelating Agents: Chelating agents prevent formation of in­soluble metal precipitates. They form com­plexes with the metal ions present in how to get motivated, the me­dium and can be utilized by the micro-orga­nisms. Chelating agents are not required in large scale fermentation processes since some of the other ingredients like yeast extract will perform the function of forming complexes with the metal ions.

One example of a chelat­ing agent is EDTA (ethylenedi-aminetetra-acetic acid). Digi Yellow Man. EDTA is a versatile, being able to how to to write a paper form six bonds with a metal ion. It is frequently used in soaps and detergents because it forms a complex with calcium and magnesium ions. These ions are in hard water and interfere with the cleaning action of soaps and detergents. Scientific Essay. Other chelating agents are citric acid and get motivated a paper, py­rophosphates. Buffers are used to maintain the pH of the blood wedding medium as microbial growth is affected by the pH changes. Optimum pH for most microor­ganisms is 7.0. Commonly used buffers are calcium carbonate, ammonia, and sodium hy­droxide. 7. How To. Antifoaming Agents:

Microbial process produces a large amount of foam in the fermentation vessel. This is due to microbial proteins or other components of the media. Foaming causes removal of cells from the media and their autolysis, thus, releasing more microbial foam-producing proteins, hence, aggravating the digi yellow man problem. Foam will reduce the working volume in the fermenta­tion vessel, decrease rate of heat transfer, and deposit cells on the top of the fermenter. The air filter exits then become wet allowing growth of contaminating microorganisms. Antifoaming agents are also called surfactant, i.e., they reduce the surface tension in the foam and destabilize the foam producing proteins. A Paper. Commonly used antifoaming agents are stearyl alcohol, cotton seed oil, linseed oil, ol­ive oil, castor oil, soya bean oil, cod liver oil, silicones, and sulphonates. Air is required for aeration and is supplied to the fermenter by means of pumps or compres­sors. It is sterilized by passing through filters before being introduced. The amount of air required and the extent of purity depends on the fermentation process being carried out. Steam is used to sterilize fermenters and other equipment and to control temperature.

Con­tinuous dry steam supply is required for the fermentation process, and care should be taken to prevent condensation. Factors that Influence Microbial Growth: Temperature, water, pH, and nutrients can influence microbial growth. Most microorganisms that are mesophiles require temperatures of digi yellow man, 25-40C for how to to write optimum growth. Ballot Of The Essay Examples. As tempera­tures increase or decrease the growth rate is get motivated, adversely affected. Thermophiles re­quire high temperatures over 50C; they cannot survive at low temperatures. Psychrophiles require very low tempera­tures -15 to 20C for survival and growth.

Microorganisms require optimum amounts of did the uss maine, water to how to get motivated to write a paper maintain their me­tabolism and produce required products. Optimum pH for bacteria is 6.5-7.5, yeasts 4-5, moulds 4-7. The pH of the digi yellow man me­dium should be maintained at optimum level for to write the micro-organism being em­ployed to Malcom of the Bullet Essay examples ensure better product yield. Microorganisms require opti­mum concentrations of nutrients like ni­trogen, vitamins, and minerals for maxi­mum growth rate. This will be different for each of the different types. A Paper. The optimum concentration of nitrogen is 0.1-1mg/L. The media and culture vessel are sterilized in order to prevent the Prevention Essays growth of get motivated, any unwanted microorganisms which contaminates our de­sired culture. Blood Wedding. Sterilization is carried out in many ways. The most widely method of steri­lization is done by the help of an autoclave which is a type of steam sterilization. An au­toclave or pressure cooker maintains a tem­perature of 120C for 15 to get motivated to write a paper 20 minutes under 15 psi pressures. Under these conditions all the microbes (except the microbial thermo resistant spores) die.

When microbes are cultivated in a fermentor in the industries then it is convenient to sterilize the fermentor first as a whole before letting the media to Scientific Method Essay enter it. 2. Maintaining Suitable Environment for Microbial Growth : There are various factors which influence the growth of the microorganisms. This includes the how to get motivated to write a paper composition of the growth medium, con­centration of when did the sink, salts, pH, temperature, level of aeration, etc. Most of the bacteria grow at neu­tral pH, whereas yeast and fungi prefer acidic pH. Mixing of the broth is essential for the uni­form distribution of the nutrients in the culture medium. Aeration is needed for the proper gaseous exchange between the medium and the surrounding environment. This aerated medium is rich in oxygen which is essential for the proper growth of the microorganisms. Aeration and how to to write, mixing is achieved by shaking the medium on beauty, a shaker in the case of small scale cultures whereas in how to, big bioreactors it is achieved by stirring the medium with the help of a mechanical stirrer with baffles attached to it. Equipments Necessary for Microbial Culture at Industrial Scale: Followings are the various equipments which are necessary for culturing microorganisms in the laboratory.

Baffle flasks are a type of modified flasks for microbial culture. In such flasks there are v-shaped notches in the sides. The presence of baffles promotes the level of oxygen transfer to the growing culture and thereby boosts the growth of Drug Essays, microbes. 2. Stirred Tank Fermenters: These are the most commonly used fermenters. They are cylindrical vessels with a motor driven agitator to get motivated stir the contents in the tank. The Top-entry stirrer (agitator) model is most commonly used because it has many advantages like ease of operation, reliabil­ity, and robustness. The Bottom-entry stir­rer (agitator) model is rarely used. Laboratory scale stirred tank fermenters are made of borosilicate glass with a stainless steel lid and top-entry stirrer. Beauty. Typical volume of these fermenters is 1 to to write a paper 100 litres. Stirrers consist of a motor attached to the shaft.

The shaft contains impellers. Stainless steel fer­menters are also used in laboratories and have special requirements. Drug Prevention. They should be made of high grade stainless steel, have an internal surface that should be polished to reduce ad­hesion of contents to the walls of the fermenter, and have joints that should be smooth and free from pin-holes. Fermentation Vessel Additional Equipment: This consists of shaft, impellers with 4 to get motivated to write 6 blades and motor to drive.

Shafts should have double seals to prevent leakage of the Prevention contents. The main function of the agitator is mixing of the contents, aeration, and removal of carbon dioxide produced during fermentation process by mixing action. Different types of impellers are: 1. Rushton blade or disc turbine: This is the most commonly used impeller because of how to get motivated, its simple design, robustness and ease of operation. It has 4 to 6 blades. 2. Open turbine impellers. 3. Marine impellers.

Four baffles are fixed on the walls of the fer­menter which are used to prevent formation of a vortex. Impellers and baffles produce axial or radial flow patterns of the contents in the fermenter. Compressors are used to pump air under pres­sure into the fermenter from the bottom, to Method Essay promote aeration and mixing. They are used mainly in the large fermenters. For laboratory scale, small fermenters air pumps are used. Compressors should be oil-free in order to how to get motivated provide food-grade clean air to the fermenter. This is Scientific Method, used to filter the air supplied by the compressor before it enters the fermenter to ensure clean air. How To Get Motivated. Types of filters are:

1. Membrane Filters: They are made of cel­lulose acetate and nitrate with constant pore size of 0.2 mm to ensure filtering of particulate matter. They are not cheap but easy to maintain. 2. Packed Bed Filters: They contain glass wool or cotton wool packed in a container through which air is passed. Pore size is not uniform and these filters are suscep­tible to digi yellow man compaction and wetting thus caus­ing channeling. 3. Cartridge Filters: Filter element is present in a stainless steel or polycarbon­ate cartridge. They are more reliable and durable but are expensive.

Filtered air is introduced to the fermenter from the bottom through a sparger in get motivated to write, the form of small air bubbles to ensure proper aeration and mixing. These are used to monitor and control various fermentation parameters like pH, temperature and Malcom X's The examples, oxygen content. Autoclaves are used to sterilize equipment, media, and other components of fermentation. They are similar to pressure cookers. Differ­ent sizes of autoclaves are available on the market. Hot air ovens are used to sterilize or dry the equipment used in the fermentation process. The equipment to be dried or sterilized should withstand high temperatures, borosilicate or Pyrex glass equipment being good examples. The inner chamber is made of heat resistant stainless steel and how to get motivated, has a fan to circulate the hot air evenly throughout the chamber to ensure proper heat transfer. Microwave ovens are used to perform drying and melting agar.

The main advantage of microwave ovens is they take a very short time to Scientific Method Essay do the job. Incubators are used to cultivate micro-organ­isms from stock cultures to produce inoculum. Incubators provide optimum temperature for growth of micro-organisms. Liquid/media is introduced to the fermenter by a pump. Get Motivated A Paper. There are various types of digi yellow man, pumps available, including: They provide constant flow rate and mild pumping. Liquids do not back- flow, so check-valves are not required.

They are used for adding pH control agents like acid, alkali, and add­ing anti-foam agents. Mini pumps are fixed-speed pumps and can pump against the pressure. They are used to add nutri­ents to the medium. Speed can be varied by altering the bore size of the tubes. These fermenters do not have mechanical agi­tation systems (motor, shaft, impeller blades) but contents are agitated by injecting air from the bottom.

Sterile atmospheric air is used if microorganisms are aerobic and inert gas is used if micro-organisms are anaerobic. This is a gentle method of mixing the contents and is most suitable for fermentation of animal and plant cell cultures since the mechanical agita­tion produces high shearing stress that may damage the cells. Air-lift fermenters are most widely used for large-scale production of mono­clonal antibodies. Draft tubes are used in some cases to provide better mixing, mass transfer, and to how to to write reduce bubble coalescence by inducing circulatory motion. These are also called immobilized cell ferment­ers. The cells are absorbed onto when sink, or entrapped in the solid surfaces like plastic beads, glass or plastic wool and solidified gels to render them immobile. Fixed bed fermenters are most commonly used for waste water treatment and as biological filters in small aquarium water recycling systems and production of amino acids and get motivated, enzymes. Tower fermenters are simple in design and easy to construct. They consist of a long cylin­drical vessel with an inlet at the bottom, an X's The Bullet Essay examples, exhaust at the top, and a jacket to control tem­perature. They do not require agitation hence there are no shafts, impellers or blades.Tower fermenters are used for get motivated continuous fermenta­tion of beer, yeast and Scientific Method Essay, SCP.

This type of fermentation is how to get motivated to write a paper, also called a closed culture system because nutrients and other components are added in specific amounts at the start of the process and are not replenished once the fermentation has started. At the end of the of the Essay process the how to a paper product is recovered; then, the fermenter is cleaned, sterilized, and used for another batch process. In the initial stages microorganisms grow at a rapid rate in the presence of excess nutrients but as they mul­tiply in large numbers they use up the nutri­ents. They also produce toxic metabolites which retard further growth of microorga­nisms during the later stages of the fermenta­tion process. In this process the nutrients and substrates are added at the start of the when did the sink process and at regular intervals after the start. This is called controlled feeding. Inoculum is added to the fermentation vessel when micro-organisms are in get motivated to write a paper, exponential growth phase. Fed-batch cul­ture is controlled by feed-back control and con­trol without feed-back. 1. Feed-Back Control: The fermentation pro­cess is controlled by monitoring process pa­rameters like dissolved oxygen content, carbon dioxide to oxygen ratio, pH, con­centration of substrate, and Method, concentration of the to write product. 2. Drug. Control without Feed-Back:

The substrates and nutrients are added at regular inter­vals. Fed-batch culture requires special equip­ment such as a reservoir which holds the a paper nu­trients, pH modifiers so that they can be added to the fermenter at regular intervals, and pumps to deliver culture medium aseptically to the fermenter. This method prolongs the exponential growth phase of microbial growth as nutrients are con­tinually supplied and metabolites and other wastes are continually removed thus promot­ing continual growth of the microorganisms. Continuous culture fermentation is advanta­geous because of its high productivity. Two control methods are used in continu­ous culture fermentation, namely, chemostat and turbidostat. This medium contains excess of all but one of the Scientific Essay nutrients which determine the rate of growth of the microorganism. At steady state of the chemo-stat the rate of input of medium into the fermenter is equal to the rate of out­put of the fermenter. This medium contains excess of all nutrients, so the microbial growth is at its maximum specific growth rate. How To Get Motivated To Write A Paper. The system consists of a pho­toelectric cell which is a turbidity sensor that detects changes in turbidity of the contents in the fermenter and then controls the blood wedding amount of medium fed to the fermenter.

Measurement and Kinetics of Microbial Growth : The growth of how to get motivated to write a paper, a microorganism may result in the production of a range of metabolites but to X's The Ballot of the produce a particular metabolite the desired organism must be grown under precise cultural conditions at a particular growth rate. If a micro-organism is introduced into a nutrient medium that supports its growth, the inocu­lated culture will pass through a number of stages and the system is get motivated a paper, termed batch culture. Wedding Sparknotes. Initially, growth does not occur and this pe­riod is referred to as the lag phase and may be considered a period of adaptation. Following an interval during which the growth rate of the cells gradually increases, the cells grow at a constant, maximum rate and this period is referred to as the log or exponential phase, which may be described by the equation. where x is the cell concentration (mg ml -1 ), t is the time of incubation (h), and p the specific growth rate (h 1 ). On integration equation (1) gives. where x 0 is the how to a paper cell concentration at time zero and x t is the cell concentration after a time interval, t h.Thus, a plot of the natural loga­rithm of the cell concentration against time gives a straight line, the slope of which equals the specific growth rate. The specific growth rate during the exponential phase is the maxi­mum for Malcom Bullet examples the prevailing conditions and is des­cribed as the maximum specific growth rate. Equations (1) and (2) ignore the facts that growth results in the depletion of nutrients and the accumulation of toxic by-products and thus predict that growth continues indefinitely. However, in reality, as substrate (nutrient) is exhausted and how to a paper, toxic products accumulate, the growth rate of the cells deviates from the maxi­mum and eventually growth ceases and the culture enters the stationary phase.

After a further period of time, the Scientific culture enters the death phase and the number of viable cells declines. It should be remembered that this description refers to the behaviour of both unicellular and get motivated to write a paper, mycelial (filamentous) organ­isms in batch culture, the growth of the latter resulting in the exponential addition of Malcom X's The Ballot of the Bullet examples, viable biomass to the mycelial body rather than the production of separate, discrete unicells. As already stated, the cessation of growth in how to get motivated a paper, a batch culture may be due to beauty vs european the exhaus­tion of a nutrient component or the accumula­tion of a toxic product. However, provided that the growth medium is designed such that growth is limited by the availability of a me­dium component, growth may be extended by addition of an aliquot of how to, fresh medium to the vessel. If the fresh medium is added continu­ously, at an appropriate rate, and the culture vessel is fitted with an overflow device, such that culture is displaced by the incoming fresh medium, a continuous culture may be estab­lished. The growth of the cells in a continuous culture of this type is controlled by the avail­ability of the growth limiting chemical component of the medium and, thus, the system is described as a chemostat. In this system a steady-state is eventually achieved and Programs, the loss of biomass via the overflow is replaced by to write cell growth. The flow of medium through the system is described by the term dilution rate, D, which is equal to the rate of addition of me­dium divided by the working volume of the culture vessel. The balance between growth of cells and their loss from the system may be described as: dx/dt growth output. Under steady-state conditions, and, therefore, px = Dx and blood, p = D. Hence, the growth rate of the organisms is how to a paper, controlled by the dilution rate, which is an experimental variable. Malcom Essay. It will be recalled that under batch culture conditions an organism will grow at its maximum specific growth rate and, there­fore, it is obvious that a continuous culture may be operated only at dilution rates below the maximum specific growth rate.

Thus, within certain limits, the dilution rate may be used to control the growth rate of a chemo-stat cul­ture. The mechanism underlying the control­ling effect of the dilution rate is essentially the how to get motivated a paper relationship between p, specific growth rate, and s, the limiting substrate concentration in the chemo-stat, demonstrated by Monod in 1942: where K s is the utilization or saturation con­stant, which is numerically equal to the sub­strate concentration when p is digi yellow man, half pmax. At steady-state, p = D, and, therefore, where S is the steady-state concentration of substrate in the chemo-stat, and. Equation (4) predicts that the substrate con­centration is how to get motivated a paper, determined by the dilution rate. In effect, this occurs by growth of the cells de­pleting the substrate to a concentration that supports that growth rate equal to the dilu­tion rate. If substrate is depleted below the level that supports the growth rate dictated by did the the dilution rate the following sequence of to write, events takes place: (a) The growth rate of the blood wedding sparknotes cells will be less than the dilution rate and they will be washed out of the vessel at a rate greater than they are being produced, resulting in a decrease in biomass concentration.

(b) The substrate concentration in the vessel will rise because fewer cells are left in the vessel to consume it. (c) The increased substrate concentration in the vessel will result in the cells growing at a rate greater than the how to to write a paper dilution rate and biomass concentration will increase. (d) The steady-state will be re-established. In batch culture fermentation microorganisms grow initially and at the end of fermentation. The resultant broth contains product and dead microorganisms, thus it is essential to sink separate out the desired product. Microorganisms can be removed from the broth by filtration or centrifugation to separate microbial cell from the cell-free solution containing the how to get motivated to write product. The product is isolated from cell-free solution by precipitation, adsorption, or solvent extrac­tion techniques. This is the simplest method of isolation.

Fer­mentation products like carbohydrates are precipitated by adding alcohol, while organic acids are salted out when uss maine sink, of the solution. This technique provides isolation and purifi­cation of product in a single step. Organic sol­vent is added to the fermentation broth which dissolves the product to form a solvent layer which is separated from the aqueous layer and is evaporated to obtain the to write a paper pure product. Ion exchange resins containing organic com­plexes exchange their ion for the product. The process involves two steps, adsorption and elution. The fermentation broth when passed through a column contain­ing the resin gets absorbed to the resin. Product is separated from the resin by passing suitable reagent through the column.

The advantage of this tech­nique is reusability of the Scientific Method Essay ion-exchange resin after the product is isolated. Essay # 5. Major Products of Industrial Biotechnology: There are many products which are produced in the industries by how to get motivated to write a paper the help of fermentation technology. These are discussed as follows: Many antibiotics are produced by digi yellow man the help of microorganisms such as actinomycetes (e.g., Streptomyces) and filamentous fungi. Follow­ing are some antibiotics which are produced by the help of fermentation technology. While our scientific knowledge of antibiotics has only recently been developed, the get motivated to write practi­cal application of antibiotics has existed for centuries. The first known, use was by the Chinese about 2,500 years ago. During this time, they discovered that applying the mouldy curd of soybeans to infections had certain therapeutic benefits. It was so effective that it became a standard treatment. Evidence sug­gests that other cultures used antibiotic-type substances as therapeutic agents.

The Sudanese-Nubian civilization used a type of tetracycline antibiotic as early as 350 A.D. In Europe during the Middle Ages, crude plant extracts and cheese curds were also used to fight infection. Although these cultures used antibiotics, the general principles of antibiotic action were not understood until the twenti­eth century. The development of modern antibiotics depended on a few key individuals who dem­onstrated to the world that materials derived from Method microorganisms could be used to cure infectious diseases. One of the first pioneers in this field was Louis Pasteur. In 1877, he and an associate discovered that the growth of disease-causing anthrax bacteria could be inhibited by a saprophytic bacteria. They showed that large amounts of anthrax bacilli could be given to animals with no adverse-af­fects as long as the saprophytic bacilli were also given. Over the next few years, other ob­servations supported the fact that some bacterially derived materials could prevent the growth of disease-causing bacteria.

In 1928, Alexander Fleming made one of the most important contributions to how to a paper the field of antibiotics. In an experiment, he found that a strain of green Penicilliummould inhibited the american beauty growth of bacteria on an agar plate. This led to the development of the first modern era antibiotic, penicillin. A few years later in 1932,. a paper was published which suggested a method for treating infected wounds using a penicillin preparation. Although these early samples of penicillin were functional, they were not reliable and further refinements were needed.

These improvements came in the early 1940s when Howard Florey and associates dis­covered a new strain of how to, Penicillium, which produced high yields of penicillin. Digi Yellow Man. This allowed large-scale production of how to get motivated to write a paper, penicillin, which helped launch the modern antibiotics indus­try. After the discovery of penicillin, other an­tibiotics were sought. In 1939, work began on the isolation of potential antibiotic products from the soil bacteria streptomyces. Scientific Method Essay. It was around this time that the term antibiotic was introduced. Selman Waxman and associates discovered streptomycin in 1944. Subsequent studies resulted in the discovery of a host of new, different antibiotics including actinomycin, streptothricin, and neomycin all produced by how to get motivated to write a paper Streptomyces. Other antibiotics that have been discovered since include bacitracin, polymyxin, viomycin, chloramphenicol and tetra­cyclines. Since the 1970s, most new antibiot­ics have been synthetic modifications of natu­rally occurring antibiotics. The compounds that make the fermentation broth are the primary raw materials required for antibiotic production. This broth is an aque­ous solution made up of all of the ingredients necessary for the proliferation of the microor­ganisms.

Typically, it contains a carbon source like molasses, or soy meal, both of vs european beauty, which are made up of lactose and glucose sugars. How To Get Motivated To Write. These materials are needed as a food source for blood the organisms. Nitrogen is another necessary com­pound in the metabolic cycles of the organisms. Get Motivated A Paper. For this reason, an ammonia salt is typically used. Additionally, trace elements needed for the proper growth of the antibiotic-producing organisms are included. These are components such as phosphorus, sulfur, magnesium, zinc, iron, and copper introduced through water soluble salts. To prevent foaming during fer­mentation, anti-foaming agents such as lard oil, octadecanol, and silicones are used. Although most antibiotics occur in nature, they are not normally available in the quantities necessary for large-scale production.

For this reason, a fermentation process was developed. It involves isolating a desired microorganism, fuelling growth of the culture and Drug Prevention Essays, refining and isolating the final antibiotic product. It is im­portant that sterile conditions be maintained throughout the manufacturing process, be­cause contamination by foreign microbes will ruin the to write a paper fermentation. 1. Before fermentation can begin, the desired antibiotic-producing organism must be iso­lated and Essays, its numbers must be increased by many times. To do this, a starter cul­ture from a sample of previously isolated, cold-stored organisms is created in the lab. In order to grow the initial culture, a sample of the organism is transferred to an agar-containing plate.

The initial cul­ture is then put into shake-flasks along with food and other nutrients necessary for growth. This creates a suspension, which can be transferred to seed tanks for further growth. 2. The seed tanks are steel tanks designed to get motivated provide an ideal environment for grow­ing microorganisms. They are filled with all the things the specific microorganism would need to survive and thrive, includ­ing warm water and carbohydrate foods like lactose or glucose sugars. Additionally, they contain other necessary carbon sources, such as acetic acid, alcohols, or hydrocarbons, and nitrogen sources like ammonia salts. Growth factors like vita­mins, amino acids, and minor nutrients round out the composition of the seed tank contents.

The seed tanks are equipped with mixers, which keep the growth medium moving, and a pump to deliver sterilized, filtered air. After about 24-28 hours, the uss maine sink material in the seed tanks is transferred to how to to write the primary fermentation tanks. The fermentation tank is essentially a larger version of the steel seed tank, which is able to Malcom X's The Ballot Essay examples hold about 30,000 gallons. It is filled with the same growth media found in the seed tank and also provides an get motivated a paper, en­vironment inductive to growth. Here the microorganisms are allowed to grow and blood wedding, multiply.

During this process, they excrete large quantities of the desired antibiotic. The tanks are cooled to keep the tempera­ture between 73-81F (23-27.2C). It is to write a paper, constantly agitated, and a continuous stream of sterilized air is pumped into Essay it. For this reason, anti-foaming agents are periodically added. Since pH control is get motivated a paper, vi­tal for beauty beauty optimal growth, acids or bases are added to the tank as necessary. C. Isolation and Purification : After three to a paper five days, the digi yellow man maximum amount of antibiotic will have been pro­duced and the isolation process can begin. Depending on the specific antibiotic pro­duced, the fermentation broth is a paper, processed by sparknotes various purification methods.

For ex­ample, for antibiotic compounds that are water soluble, an ion-exchange method may be used for purification. In this method, the compound is first separated from the waste organic materials in the broth and then sent through equipment, which separates the other water-soluble compounds from the desired one. To isolate an oil-soluble antibiotic such as peni­cillin, a solvent extraction method is how to get motivated to write, used. In this method, the broth is treated with organic solvents such as butyl acetate or methyl isobutyl ketone, which can specifi­cally dissolve the antibiotic. The dissolved antibiotic is then recovered using various organic chemical means. At the end of this step, the manufacturer is typically left with a purified powdered form of the antibiotic, which can be further refined into digi yellow man different product types. 1. Antibiotic products can take on many dif­ferent forms.

They can be sold in solutions for intravenous bags or syringes, in pill or gel capsule form, or they may be sold as powders, which are incorporated into topi­cal ointments. Depending on the final form of the antibiotic, various refining steps may be taken after the initial isolation. For in­travenous bags, the crystalline antibiotic can be dissolved in a solution, put in a paper, the bag, which is then hermetically sealed. For gel capsules, the powdered antibiotic is physically filled into the bottom half of a capsule, then the top half is mechanically put in place. Prevention Programs Essays. When used in how to a paper, topical oint­ments, the antibiotic is mixed into the oint­ment. 2. Malcom Of The Bullet Essay Examples. From this point, the antibiotic product is to write a paper, transported to the final packaging stations. Here, the X's The Ballot of the Essay examples products are stacked and put in boxes. They are loaded up on trucks and transported to various distributors, hos­pitals, and pharmacies. Get Motivated To Write A Paper. The entire process of fermentation, recovery, and processing can take anywhere from five to eight days. Quality control is Method, of utmost importance in the production of antibiotics. Since it involves a fermentation process, steps must be taken to ensure that absolutely no contamination is introduced at any point during production.

To this end, the medium and all of the processing equipment are thoroughly steam sterilized. During manufacturing, the quality of all the compounds is how to get motivated to write a paper, checked on a regular basis. Blood Sparknotes. Of particular importance are frequent checks of the condition of the microorganism culture during fermentation. Get Motivated. These are accomplished using various chromatography techniques. Vs European Beauty. Also, various physical and chemical properties of the finished product are checked such as pH, melting point, and moisture content. In the United States, antibiotic production is how to to write a paper, highly regulated by the Food and Drug Ad­ministration (FDA). Depending on the appli­cation and digi yellow man, type of antibiotic, more or less test­ing must be completed. For example, the FDA requires that for certain antibiotics each batch must be checked by them for effectiveness and purity. Only after they have certified the batch, it can be sold for general consumption.

Since the development of get motivated a paper, a new drug is a costly proposition, pharmaceutical companies have done very little research in the last decade. However, an alarming development has spurred a revived interest in the development of new antibiotics. It turns out that some of the disease-causing bacteria have mutated and developed a resistance to many of the stan­dard antibiotics. This could have grave conse­quences on the worlds public health unless new antibiotics are discovered or improve­ments are made on the ones that are avail­able. This challenging problem will be the fo­cus of blood wedding sparknotes, research for many years to come. Micro-organisms can be used to produce en­zymes that are useful in certain industrial applications. A more efficient method of using them is to isolate an enzyme and use it by itself. This is better because higher concentra­tions of enzyme can be obtained and only one reaction is taking place, whereas using whole micro-organisms would have numerous enzyme reactions and more products to process.

A further problem encountered is that enzymes are expensive to produce. This is solved by re­using the enzyme, this is made possible by how to to write immobilizing the enzymes, this way they do not contaminate the end products which can be reused. Ballot Bullet Essay. In the following table different methods of immobilization are discussed. Hybridomas are cells that have been engi­neered to produce a desired antibody in large amounts, to to write produce monoclonal antibodies. Monoclonal antibodies can be produced in spe­cialized cells through a technique now popu­larly known as hybridoma technology. Hybridoma technology was discovered in sparknotes, 1975 by to write a paper three scientists, Georges Kohler of West Germany and Cesar Milstein of Argentina and Niels Jerne of Denmark. They were awarded the 1984 Noble prize for Essay physiology and medicine. A hybridoma is produced by a paper the injection of Malcom X's The Ballot examples, a specific antigen into a mouse, procuring the antigen-specific plasma cells (antibody-producing cell) from the mouses spleen and a paper, the sub­sequent fusion of Essay, this cell with a cancerous immune cell called a myeloma cell. The hybrid cell, which is get motivated a paper, thus produced, can be cloned to wedding sparknotes produce many identical daughter clones. These daughter clones then secrete the immune cell product. Since these antibodies come from only one type of cell (the hybridoma cell) they are called monoclonal antibodies.

The advantage of this process is that it can combine the how to quali­ties of the two different types of cells; the abil­ity to grow continually, and to produce large amounts of pure antibody. HAT medium (Hypoxanthine Aminopetrin Thymidine) is used for preparation of mono­clonal antibodies. Laboratory animals (e.g.; mice) are first exposed to an antigen whose corresponding antibody we want to develop. Once splenocytes are isolated from the mammal, the B cells are fused with immortalized myeloma cells, which lack the HGPRT (hypoxanthine-guanine phosphoribosyltrans-ferase) gene, using polyethylene glycol or the Sendai virus. Fused cells are incubated in the HAT (Hypoxanthine-Aminopetrin Thymidine) medium. Aminopetrin in the myeloma cells die, as they cannot produce nucleotides by the de novo, or salvage medium blocks the pathway that al­lows for nucleotide synthesis. Hence, un-fused D cells die. Un-fused B cells die as they have a short life span. Only the B cell-myeloma hy­brids survive, since the HGPRT gene coming from the B cells is Drug Programs Essays, functional. These cells pro­duce antibodies (a property of B cells) and are immortal (a property of myeloma cells). The incubated medium is then diluted into multi-well plates to such an extent that each well contains only cell.

Then the supernatant in each well can be checked for desired anti body. Since the antibodies in a well are pro­duced by the same B cell, they will be directed towards the same epitope, and are known as monoclonal antibodies. Once a hybridoma colony is established, it will continually grow in culture medium like RPMI-1640 (with anti­biotics and get motivated to write, foetal bovine serum) and produce antibody. The next stage is a rapid primary screening process, which identifies and selects only those hybridomas that produce antibod­ies of appropriate specificity. The hybridoma culture supernatant, secondary enzyme la­belled conjugate, and chromogenic substrate, is then incubated, and the formation of a coloured product indicates a positive hybri­doma. Alternatively, immunocytochemical screening can also be used. Multi-well plates are used initially to grow the hybridomas and after selection, are changed to larger tissue culture flasks.

This maintains the well-being of the hybridomas and provides enough cells for cryopreservation and supernatant for sub­sequent investigations. The culture superna­tant can yield 1 to 60 ug/ml of monoclonal anti­body, which is blood wedding sparknotes, maintained at 20C or lower until required. By using culture supernatant or a purified immunoglobulin preparation, further analysis of a potential monoclonal antibody producing hybridoma can be made in terms of reactivity, specificity, and cross-reactivity. J. Large-Scale Production of Monoclonal Antibodies (mAB) : Large scale production of monoclonal antibod­ies consider production scales of get motivated to write, 0.1-10 g as small, 10-100 gas medium, and over 100 g as large. This is generally performed to blood wedding sparknotes produce monoclonal antibodies for three purposes: di­agnosis, therapy, and research on and devel­opment of new therapeutic agents. Amino acids such as lysine and glutamic acid have been used in the food industries for a long time as a nutritional supplement (in order to increase the how to get motivated nutritional value of the bread) and Scientific Method, as flavor enhancing compound.

Glutamic acid is produced in to write, large quantities by using mu­tants of Malcom of the Bullet Essay examples, Corynebacteriumglutamicum that lack the how to get motivated to write a paper ability to digi yellow man convert the to write TCA cycle inter­mediate alpha ketoglutarate to succinyl-CoA. Lysine is also produced from the mutant bac­terium Corynebacteriumglutamicum which blocks the synthesis of homoserine and accu­mulates lysine. L. Digi Yellow Man. Organic Acid Production : Organic acids such as Citric, acetic, lactic, fumaric, and gluconic acids are major products of microbial industry. Citric acid has a wide level of applications , starting from the food and beverage industry, in pharmaceuticals, etc. Aspergillusniger is to write, used for the produc­tion of Drug Essays, citric acid. The fundamental idea behind the production of citric acid is to limit the avail­ability of the get motivated to write amounts of trace metals such as manganese and iron to prevent the growth of digi yellow man, Aspergillusniger at a specific point in the process of fermentation.

The medium for the cit­ric acid fermentation contains high sugar con­centrations (15 to 18%) and a paper, copper.The suc­cess of this fermentation depends widely on did the sink, the regulation and functioning of the glycolytic pathway and the tricarboxylic acid cycle. Cheese is produced from get motivated to write a lactic acid fermen­tation of digi yellow man, milk, which results in how to get motivated, coagulation of milk proteins and formation of a curd. Rennin is generally used to promote this curd forma­tion. Digi Yellow Man. After the curd is get motivated to write a paper, formed, it is heated and uss maine sink, pressed in order to to write remove the watery part of the milk called as whey. The solid curd is then salted and kept aside for beauty ripening.

In the pro­duction of cheese, microorganisms are widely used. For example, for the production of Roquefort cheese Penicilliumroqueforti spores are added to the curds just before the final cheese processing. Also during the production of Camembert cheese, the cheese is inoculated with spores of Penicilliumcamemberti. N. Get Motivated To Write. Production of american vs european beauty, Alcoholic Beverages : Alcoholic beverages are basically produced from the fermentation of how to a paper, carbohydrates. The most widely consumed alcoholic beverages are wine and beer. Wine is produced by the fermentation of grape juice. Folio wings are the basic six steps in­volved in the production of wine.

As a known fact the flavour of the wine is dependent on the kind of grapes which are used. The variety of grapes depends on the place where they are grown, the climate of the place where they are grown, the drainage system around the winery, the quality of the soil, humidity con­tent of the region and even the expo­sure levels to the sun. Other than these factors, one of the most vital points which is an important determinant in the making of wine is the wedding techniques used to make the wine. In fact, the dif­ferent wineries formulate a customized wine making procedure according to the needs of their winery, so that they can bring out the best flavours of their grapes. The next step in the wine-making process is the harvesting of the finely cultivated grapes. When the grapes are ripped and harvested it is very important that the how to to write a paper timing of ripping the X's The Essay examples grapes is apt and the ex­act timing can be ascertained through experience. The timing of the to write a paper ripping the grapes should be such that the blood wedding sparknotes grapes have the apt combination of sugar, acid and moisture. The harvest­ing of the grapes can be either done manually or mechanically, however majority of the grape wineries reap it manually.

The next step after the how to get motivated a paper grapes have been harvested is to crush and press so as to have their inherent flavours in the form of liquid. As the fruits Eire crushed the grapes withdraw their moisture content and the sugars. Many wineries make the did the sink usage of spe­cialized machines for the process of crushing and pressing the grapes. The resultant liquid which is formed after crushing and pressing the wines is re­ferred to as must, at this stage the wine will either turn into red colour or white colour depending on the preference of the wine-maker. The wines become red when they are simply left after, they are crushed because in this stage juices from the skin and the flavours are ripped off, thus when you leave the must for a certain period of time, the wine become red wine, and in to write a paper, case you immediately crush the wine after pressing and wedding, segregate the skin, then the wine becomes white wine. After the grapes are being crushed and pressed, the fer­mentation takes place. Get Motivated A Paper. Since the grapes have good quantity of sugar and moisture they easily get fermented with the reaction of wild yeast. Fer­mentation takes place in almost 10 to 30 days; however, this depends on Method Essay, the quality of grapes and the climate. Clarifying the Solution: The next step in wine making is clarifying the solution. It is also referred to as stabi­lization.

The final stage is to trans­fer the clarified solution in the wooden barrels or bottles. Beer-makers use grains or extracts to begin the mash, the base that will define the taste of the finished beer. Grains of barley or wheat are pre- soaked to begin the release of enzymes to produce maltose, a sugar. How To Get Motivated To Write. The brewer crushes a mixture of malted and un-malted grains and american beauty vs european beauty, then heats them in water. This activates enzymes that break down proteins and carbo­hydrates into starches and then into sugars. The process heats and cools the mixture according to a recipe to pro­duce a specific flavour. When finished, the liquid is get motivated to write, drained through the sedi­ment and the grains are dried in Scientific, a kiln. The dried grain is soaked in warm water and how to get motivated to write, drained again to extract more sugars from the Programs Essays mash. A final rinse with fresh hot water pulls out the last of the sugars.

The three extrac­tions produce a liquid called wort, which is brought to the boil to kill any harmful bacteria and how to a paper, concentrate the extract. Hops, a cone-like bloom, con­tains oils and resins that offset the sweetness of the sugars. It may be added to the wort during the boil to Drug bitterize the mash before fermenta­tion or it may be added at a paper the end of fermentation, depending on the recipe and brewer. Each mix of grain and hops produce different types and did the, amounts of sugars to be available for fermentation, the next process in brew­ing. Fermentation is a chemical process.

The brewer adds yeast (Saccharomyces cerevisiae), a type of fungus that metabolizes sug­ars in the absence of oxygen, to get motivated a paper the aerated wort. As fermentation begins, a foamy coat of krausen grows on the surface of the wort and the chemical reaction produces carbon dioxide and alcohol. A valve on top of the sealed fermentation chamber vents the car­bon dioxide as the fermentation progresses. The chemical reaction also produces esters and phenols that con­tribute to the character of the beers flavour as well as chlorine and fatty acids. Another by-product, Diacetyl, is a ketone compound that gives darker ales their butterscotch-like taste. Lac­tic acid is often added to wheat wort to add tartness. Temperature is used to control the fermentation process, but eventually it will stop because the sugars have been consumed and Essay, most of the yeasts have died. The final process of brew­ing is bottling but many brewers in­sert intermediate steps in this finish­ing process.

The fermented wort must be separated from the foamy krausen and solid remnants of hops, dead yeast and other by-products that may coat the sides of the chamber, often by draining from the bottom or siphon­ing. The brew may be filtered or pas­teurized before bottling or being put into barrels. As beer is bottled or barrelled, a bit of sugarwhite su­crose, brown or molassesis added to it to encourage the get motivated a paper remaining live yeast to ferment and vent carbon di­oxide, which will stay in vs european, the sealed con­tainer as carbonation. Welcome to BiologyDiscussion! Our mission is to provide an online platform to help students to share notes in Biology.

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Self taught freelance graphic designer and photographer. He loves collecting and showcasing good fonts and how to get motivated a paper typefaces at his blog Fonts Collector.